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dc.contributor.authorHausammann, Georg J.-
dc.contributor.authorHeitkamp, Thomas-
dc.contributor.authorMatile, Hugues-
dc.contributor.authorGsell, Bernard-
dc.contributor.authorThoma, Ralf-
dc.contributor.authorSchmid, Georg-
dc.contributor.authorFrasson, David-
dc.contributor.authorSievers, Martin-
dc.contributor.authorHennig, Michael-
dc.contributor.authorGrütter, Markus G.-
dc.date.accessioned2018-10-19T14:13:51Z-
dc.date.available2018-10-19T14:13:51Z-
dc.date.issued2013-
dc.identifier.issn0006-291Xde_CH
dc.identifier.issn1090-2104de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/11991-
dc.description.abstractThe human ether-a-go-go related gene (hERG) potassium channel plays a major role in the repolarization of the cardiac action potential. Inhibition of the hERG function by mutations or a wide variety of pharmaceutical compounds cause long QT syndrome and lead to potentially lethal arrhythmias. For detailed insights into the structural and biochemical background of hERG function and drug binding, the purification of recombinant protein is essential. Because the hERG channel is a challenging protein to purify, fast and easy techniques to evaluate different expression, solubilization and purification conditions are of primary importance. Here, we describe the generation of a set of 12 monoclonal antibodies against hERG. Beside their suitability in western blot, immunoprecipitation and immunostaining, these antibodies were used to establish a sandwich ELISA for the detection and relative quantification of hERG in different expression systems. Furthermore, a Fab fragment was used in fluorescence size exclusion chromatography to determine the oligomeric state of hERG after solubilization. These new tools can be used for a fast and efficient screening of expression, solubilization and purification conditions.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofBiochemical and Biophysical Research Communicationsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectMonoclonal antibodiesde_CH
dc.subjectGel chromatographyde_CH
dc.subjectERG1 potassium channelde_CH
dc.subjectEther-A-Go-Go potassium channelsde_CH
dc.subjectHEK293 cellsde_CH
dc.subjectEnzyme-linked immunosorbent assayde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.titleGeneration of an antibody toolbox to characterize hERGde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.bbrc.2012.12.089de_CH
dc.identifier.pmid23277102de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end75de_CH
zhaw.pages.start70de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume431de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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