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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sievers, Martin | - |
dc.contributor.author | Uermösi, Christina | - |
dc.contributor.author | Fehlmann, Marc | - |
dc.contributor.author | Krieger, Sibylle | - |
dc.date.accessioned | 2018-10-19T14:15:31Z | - |
dc.date.available | 2018-10-19T14:15:31Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0723-2020 | de_CH |
dc.identifier.issn | 1618-0984 | de_CH |
dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/11993 | - |
dc.description.abstract | The nucleotide sequences of the genes encoding the F1F0-ATPase beta-subunit from Oenococcus oeni, Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus damnosus, Pediococcus parvulus, Lactobacillus brevis and Lactobacillus hilgardii were determined. Their deduced amino acid sequences showed homology values of 79-98%. Data from the alignment and ATPase tree indicated that O. oeni and L. mesenteroides subsp. mesenteroides formed a group well-separated from P. damnosus and P. parvulus and from the group comprises L. brevis and L. hilgardii. The N-terminus of the F1F0-ATPase beta-subunit of O. oeni contains a stretch of additional 38 amino acid residues. The catalytic site of the ATPase beta-subunit of the investigated strains is characterized by the two conserved motifs GGAGVGKT and GERTRE. The amplified atpD coding sequences were inserted into the pCRT7/CT-TOPO vector using TA-cloning strategy and transformed in Escherichia coli. SDS-PAGE and Western blot analyses confirmed that O. oeni has an ATPase beta-subunit protein which is larger in size than the corresponding molecules from the investigated strains. | de_CH |
dc.language.iso | en | de_CH |
dc.publisher | Elsevier | de_CH |
dc.relation.ispartof | Systematic and Applied Microbiology | de_CH |
dc.rights | Licence according to publishing contract | de_CH |
dc.subject | Oenococcus oeni | de_CH |
dc.subject | Atpase | de_CH |
dc.subject | Wine lactic acid bacteria | de_CH |
dc.subject.ddc | 570: Biologie | de_CH |
dc.title | Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria | de_CH |
dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
dcterms.type | Text | de_CH |
zhaw.departement | Life Sciences und Facility Management | de_CH |
zhaw.organisationalunit | Institut für Chemie und Biotechnologie (ICBT) | de_CH |
dc.identifier.doi | 10.1078/072320203322497374 | de_CH |
dc.identifier.pmid | 14529177 | de_CH |
zhaw.funding.eu | No | de_CH |
zhaw.issue | 3 | de_CH |
zhaw.originated.zhaw | Yes | de_CH |
zhaw.pages.end | 356 | de_CH |
zhaw.pages.start | 350 | de_CH |
zhaw.publication.status | publishedVersion | de_CH |
zhaw.volume | 26 | de_CH |
zhaw.publication.review | Peer review (Publikation) | de_CH |
zhaw.webfeed | Mikrobiologie | de_CH |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Sievers, M., Uermösi, C., Fehlmann, M., & Krieger, S. (2003). Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology, 26(3), 350–356. https://doi.org/10.1078/072320203322497374
Sievers, M. et al. (2003) ‘Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria’, Systematic and Applied Microbiology, 26(3), pp. 350–356. Available at: https://doi.org/10.1078/072320203322497374.
M. Sievers, C. Uermösi, M. Fehlmann, and S. Krieger, “Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria,” Systematic and Applied Microbiology, vol. 26, no. 3, pp. 350–356, 2003, doi: 10.1078/072320203322497374.
SIEVERS, Martin, Christina UERMÖSI, Marc FEHLMANN und Sibylle KRIEGER, 2003. Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology. 2003. Bd. 26, Nr. 3, S. 350–356. DOI 10.1078/072320203322497374
Sievers, Martin, Christina Uermösi, Marc Fehlmann, and Sibylle Krieger. 2003. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology 26 (3): 350–56. https://doi.org/10.1078/072320203322497374.
Sievers, Martin, et al. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology, vol. 26, no. 3, 2003, pp. 350–56, https://doi.org/10.1078/072320203322497374.
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