Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria

Sievers, Martin; Uermösi, Christina; Fehlmann, Marc; Krieger, Sibylle

doi:10.1078/072320203322497374

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria
Authors:	Sievers, Martin Uermösi, Christina Fehlmann, Marc Krieger, Sibylle
DOI:	10.1078/072320203322497374
Published in:	Systematic and Applied Microbiology
Volume(Issue):	26
Issue:	3
Page(s):	350
Pages to:	356
Issue Date:	2003
Publisher / Ed. Institution:	Elsevier
ISSN:	0723-2020 1618-0984
Language:	English
Subjects:	Oenococcus oeni; Atpase; Wine lactic acid bacteria
Subject (DDC):	570: Biology
Abstract:	The nucleotide sequences of the genes encoding the F1F0-ATPase beta-subunit from Oenococcus oeni, Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus damnosus, Pediococcus parvulus, Lactobacillus brevis and Lactobacillus hilgardii were determined. Their deduced amino acid sequences showed homology values of 79-98%. Data from the alignment and ATPase tree indicated that O. oeni and L. mesenteroides subsp. mesenteroides formed a group well-separated from P. damnosus and P. parvulus and from the group comprises L. brevis and L. hilgardii. The N-terminus of the F1F0-ATPase beta-subunit of O. oeni contains a stretch of additional 38 amino acid residues. The catalytic site of the ATPase beta-subunit of the investigated strains is characterized by the two conserved motifs GGAGVGKT and GERTRE. The amplified atpD coding sequences were inserted into the pCRT7/CT-TOPO vector using TA-cloning strategy and transformed in Escherichia coli. SDS-PAGE and Western blot analyses confirmed that O. oeni has an ATPase beta-subunit protein which is larger in size than the corresponding molecules from the investigated strains.
URI:	https://digitalcollection.zhaw.ch/handle/11475/11993
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Sievers, M., Uermösi, C., Fehlmann, M., & Krieger, S. (2003). Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology, 26(3), 350–356. https://doi.org/10.1078/072320203322497374

Sievers, M. et al. (2003) ‘Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria’, Systematic and Applied Microbiology, 26(3), pp. 350–356. Available at: https://doi.org/10.1078/072320203322497374.

M. Sievers, C. Uermösi, M. Fehlmann, and S. Krieger, “Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria,” Systematic and Applied Microbiology, vol. 26, no. 3, pp. 350–356, 2003, doi: 10.1078/072320203322497374.

SIEVERS, Martin, Christina UERMÖSI, Marc FEHLMANN und Sibylle KRIEGER, 2003. Cloning, sequence analysis and expression of the F1F0- ATPase beta-subunit from wine lactic acid bacteria. Systematic and Applied Microbiology. 2003. Bd. 26, Nr. 3, S. 350–356. DOI 10.1078/072320203322497374

Sievers, Martin, Christina Uermösi, Marc Fehlmann, and Sibylle Krieger. 2003. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology 26 (3): 350–56. https://doi.org/10.1078/072320203322497374.

Sievers, Martin, et al. “Cloning, Sequence Analysis and Expression of the F1F0- ATPase Beta-Subunit from Wine Lactic Acid Bacteria.” Systematic and Applied Microbiology, vol. 26, no. 3, 2003, pp. 350–56, https://doi.org/10.1078/072320203322497374.