|Publication type:||Article in scientific journal|
|Type of review:||Not specified|
|Title:||Robust hit identification by quality assurance and multivariate data analysis of a high-content, cell-based assay|
|Published in:||Journal of Biomolecular Screening|
|Publisher / Ed. Institution:||Sage|
|Subjects:||Cluster analysis; Multivariate analysis; Neurites; Quality control; Reproducibility of results; Tissue array analysis|
|Subject (DDC):||572: Biochemistry|
|Abstract:||Recent technological advances in high-content screening instrumentation have increased its ease of use and throughput, expanding the application of high-content screening to the early stages of drug discovery. However, high-content screens produce complex data sets, presenting a challenge for both extraction and interpretation of meaningful information. This shifts the high-content screening process bottleneck from the experimental to the analytical stage. In this article, the authors discuss different approaches of data analysis, using a phenotypic neurite outgrowth screen as an example. Distance measurements and hierarchical clustering methods lead to a profound understanding of different high-content screening readouts. In addition, the authors introduce a hit selection procedure based on machine learning methods and demonstrate that this method increases the hit verification rate significantly (up to a factor of 5), compared to conventional hit selection based on single readouts only.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||School of Engineering|
|Organisational Unit:||Institute of Data Analysis and Process Design (IDP)|
|Appears in collections:||Publikationen School of Engineering|
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Dürr, O., Duval, F., Nichols, A., Lang, P., Brodte, A., Heyse, S., & Besson, D. (2007). Robust hit identification by quality assurance and multivariate data analysis of a high-content, cell-based assay. Journal of Biomolecular Screening, 12(8), 1042–1049. https://doi.org/10.1177/1087057107309036
Dürr, O. et al. (2007) ‘Robust hit identification by quality assurance and multivariate data analysis of a high-content, cell-based assay’, Journal of Biomolecular Screening, 12(8), pp. 1042–1049. Available at: https://doi.org/10.1177/1087057107309036.
O. Dürr et al., “Robust hit identification by quality assurance and multivariate data analysis of a high-content, cell-based assay,” Journal of Biomolecular Screening, vol. 12, no. 8, pp. 1042–1049, 2007, doi: 10.1177/1087057107309036.
Dürr, Oliver, et al. “Robust Hit Identification by Quality Assurance and Multivariate Data Analysis of a High-Content, Cell-Based Assay.” Journal of Biomolecular Screening, vol. 12, no. 8, 2007, pp. 1042–49, https://doi.org/10.1177/1087057107309036.
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