Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-18117
Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types |
Authors: | Born, Yannick Knecht, Leandra E. Eigenmann, Mirjam Bolliger, Michel Klumpp, Jochen Fieseler, Lars |
et. al: | No |
DOI: | 10.1007/s00705-019-04148-6 10.21256/zhaw-18117 |
Published in: | Archives of Virology |
Volume(Issue): | 164 |
Issue: | 3 |
Page(s): | 819 |
Pages to: | 830 |
Issue Date: | 2019 |
Publisher / Ed. Institution: | Springer |
ISSN: | 0304-8608 1432-8798 |
Language: | English |
Subjects: | Bacteriophage; Capsid protein; Erwinia amylovora; Escherichia coli; Multiplex polymerase chain reaction; Phylogeny; Salmonella enterica; Virulence |
Subject (DDC): | 570: Biology |
Abstract: | Bacteriophages represent a promising alternative for controlling pathogenic bacteria. They are ubiquitous in the environment, and their isolation is usually simple and fast. However, not every phage is suitable for biocontrol applications. It must be virulent (i.e., strictly lytic), non-transducing, and safe. We have developed a method for identifying selected types of virulent phages at an early stage of the isolation process to simplify the search for suitable candidates. Using the major capsid protein (MCP) as a phylogenetic marker, we designed degenerate primers for the identification of Felix O1-, GJ1-, N4-, SP6-, T4-, T7-, and Vi1-like phages in multiplex PCR setups with single phage plaques as templates. Performance of the MCP PCR assay was evaluated with a set of 26 well-characterized phages. Neither false-positive nor false-negative results were obtained. In addition, 154 phages from enrichment cultures from various environmental samples were subjected to MCP PCR analysis. Eight of them, specific for Salmonella enterica, Escherichia coli, or Erwinia amylovora, belonged to one of the selected phage types. Their PCR-based identification was successfully confirmed by pulsed-field gel electrophoresis of the phage genomes, electron microscopy, and sequencing of the amplified mcp gene fragment. The MCP PCR assay was shown to be a simple method for preliminary assignment of new phages to a certain group and thus to identify candidates for biocontrol immediately after their isolation. Given that sufficient sequence data are available, this method can be extended to any phage group of interest. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/18117 |
Fulltext version: | Published version |
License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Food and Beverage Innovation (ILGI) |
Published as part of the ZHAW project: | Mechanisms of phage resistance in Erwinia amylovora |
Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
File | Description | Size | Format | |
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Born2019_Article_AMajor-capsid-protein-basedMul.pdf | 1.43 MB | Adobe PDF | View/Open |
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Born, Y., Knecht, L. E., Eigenmann, M., Bolliger, M., Klumpp, J., & Fieseler, L. (2019). A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types. Archives of Virology, 164(3), 819–830. https://doi.org/10.1007/s00705-019-04148-6
Born, Y. et al. (2019) ‘A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types’, Archives of Virology, 164(3), pp. 819–830. Available at: https://doi.org/10.1007/s00705-019-04148-6.
Y. Born, L. E. Knecht, M. Eigenmann, M. Bolliger, J. Klumpp, and L. Fieseler, “A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types,” Archives of Virology, vol. 164, no. 3, pp. 819–830, 2019, doi: 10.1007/s00705-019-04148-6.
BORN, Yannick, Leandra E. KNECHT, Mirjam EIGENMANN, Michel BOLLIGER, Jochen KLUMPP und Lars FIESELER, 2019. A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types. Archives of Virology. 2019. Bd. 164, Nr. 3, S. 819–830. DOI 10.1007/s00705-019-04148-6
Born, Yannick, Leandra E. Knecht, Mirjam Eigenmann, Michel Bolliger, Jochen Klumpp, and Lars Fieseler. 2019. “A Major-Capsid-Protein-Based Multiplex PCR Assay for Rapid Identification of Selected Virulent Bacteriophage Types.” Archives of Virology 164 (3): 819–30. https://doi.org/10.1007/s00705-019-04148-6.
Born, Yannick, et al. “A Major-Capsid-Protein-Based Multiplex PCR Assay for Rapid Identification of Selected Virulent Bacteriophage Types.” Archives of Virology, vol. 164, no. 3, 2019, pp. 819–30, https://doi.org/10.1007/s00705-019-04148-6.
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