Qualitative microbiome profiling along a wastewater system in Kampala, Uganda

Schneeberger, Pierre H. H.; Fuhrimann, Samuel; Becker, Sören L.; Pothier, Joël F.; Duffy, Brion; Beuret, Christian; Frey, Jürg E.; Utzinger, Jürg

doi:10.1038/s41598-019-53569-5

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-18805

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
Authors:	Schneeberger, Pierre H. H. Fuhrimann, Samuel Becker, Sören L. Pothier, Joël F. Duffy, Brion Beuret, Christian Frey, Jürg E. Utzinger, Jürg
et. al:	No
DOI:	10.1038/s41598-019-53569-5 10.21256/zhaw-18805
Published in:	Scientific Reports
Volume(Issue):	9
Issue:	1
Page(s):	17334
Issue Date:	22-Nov-2019
Publisher / Ed. Institution:	Nature Publishing Group
ISSN:	2045-2322
Language:	English
Subjects:	Fresh water ecology; Water microbiology; Wetlands ecology
Subject (DDC):	570: Biology 577: Ecology
Abstract:	Kampala, the capital city of Uganda, is rapidly expanding without adequate wastewater treatment facilities to accommodate the current estimated population of 1.68 million people. Hence, freshwater bodies and natural ecosystems around the city are heavily polluted with organic and inorganic contaminants. Yet, there is a paucity of data on pathogenic microorganisms, which potentially threatens health of local communities. We performed a qualitative microbial analysis using a whole metagenome sequencing approach encompassing over 150 gigabases of sequencing data to characterize the Nakivubo wastewater system, which includes a wastewater channel and surrounding wetlands. We found that microbial diversity is heterogeneous throughout the system and that three community state types could be differentiated. We showed the presence of various waterborne agents of gastrointestinal infections in humans, which were associated with leakage occurring around two locations along the wastewater channel. Our data indicate that the microbial decontamination capacity of the local wastewater treatment facility was insufficient at the time of sampling, and that several areas of the wetlands were contaminated with human pathogens, indicating that parts of the wetlands are potentially unsafe for urban agriculture.
URI:	https://digitalcollection.zhaw.ch/handle/11475/18805
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	In-depth molecular analysis of stool and water samples collected in Kampala, Uganda
Appears in collections:	Publikationen Life Sciences und Facility Management

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Schneeberger, P. H. H., Fuhrimann, S., Becker, S. L., Pothier, J. F., Duffy, B., Beuret, C., Frey, J. E., & Utzinger, J. (2019). Qualitative microbiome profiling along a wastewater system in Kampala, Uganda. Scientific Reports, 9(1), 17334. https://doi.org/10.1038/s41598-019-53569-5

Schneeberger, P.H.H. et al. (2019) ‘Qualitative microbiome profiling along a wastewater system in Kampala, Uganda’, Scientific Reports, 9(1), p. 17334. Available at: https://doi.org/10.1038/s41598-019-53569-5.

P. H. H. Schneeberger et al., “Qualitative microbiome profiling along a wastewater system in Kampala, Uganda,” Scientific Reports, vol. 9, no. 1, p. 17334, Nov. 2019, doi: 10.1038/s41598-019-53569-5.

SCHNEEBERGER, Pierre H. H., Samuel FUHRIMANN, Sören L. BECKER, Joël F. POTHIER, Brion DUFFY, Christian BEURET, Jürg E. FREY und Jürg UTZINGER, 2019. Qualitative microbiome profiling along a wastewater system in Kampala, Uganda. Scientific Reports. 22 November 2019. Bd. 9, Nr. 1, S. 17334. DOI 10.1038/s41598-019-53569-5

Schneeberger, Pierre H. H., Samuel Fuhrimann, Sören L. Becker, Joël F. Pothier, Brion Duffy, Christian Beuret, Jürg E. Frey, and Jürg Utzinger. 2019. “Qualitative Microbiome Profiling along a Wastewater System in Kampala, Uganda.” Scientific Reports 9 (1): 17334. https://doi.org/10.1038/s41598-019-53569-5.

Schneeberger, Pierre H. H., et al. “Qualitative Microbiome Profiling along a Wastewater System in Kampala, Uganda.” Scientific Reports, vol. 9, no. 1, Nov. 2019, p. 17334, https://doi.org/10.1038/s41598-019-53569-5.