| Publication type: | Book part |
| Type of review: | Editorial review |
| Title: | How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale |
| Authors: | Schirmer, Cedric Müller, Jan Steffen, Nina Werner, Sören Eibl-Schindler, Regine Eibl, Dieter |
| et. al: | No |
| DOI: | 10.1007/978-1-0716-0191-4_10 |
| Published in: | Animal cell biotechnology : methods and protocols |
| Editors of the parent work: | Pörtner, Ralf |
| Page(s): | 169 |
| Pages to: | 186 |
| Issue Date: | 2020 |
| Series: | Methods in Molecular Biology |
| Series volume: | 2095 |
| Publisher / Ed. Institution: | Springer |
| Publisher / Ed. Institution: | New York |
| ISBN: | 978-1-0716-0190-7 978-1-0716-0191-4 |
| Language: | English |
| Subjects: | CHO cell expansion; Fed-batch; IgG production; Stirred single-use bioreactor; Wave-mixed single-use bioreactor |
| Subject (DDC): | 660: Chemical engineering |
| Abstract: | Single-use bioreactors have increasingly been used in recent years, for both research and development as well as industrial production, especially in mammalian cell-based processes. Among the numerous single-use bioreactors available today, wave-mixed bags and stirred systems dominate. Wave-mixed single-use bioreactors are the system of choice for inoculum production, while stirred single-use bioreactors are most often preferred for antibody expression. For this reason, the present chapter describes protocols instructing the reader to use the wave-mixed BIOSTAT® RM 50 for cell expansion and to produce a monoclonal antibody (mAb) in Pall's Allegro™ STR 200 at pilot scale for the first time. All methods described are based on a Chinese hamster ovary (CHO) suspension cell line expressing a recombinant immunoglobulin G (IgG). |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/19321 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Schirmer, C., Müller, J., Steffen, N., Werner, S., Eibl-Schindler, R., & Eibl, D. (2020). How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale. In R. Pörtner (Ed.), Animal cell biotechnology : methods and protocols (pp. 169–186). Springer. https://doi.org/10.1007/978-1-0716-0191-4_10
Schirmer, C. et al. (2020) ‘How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale’, in R. Pörtner (ed.) Animal cell biotechnology : methods and protocols. New York: Springer, pp. 169–186. Available at: https://doi.org/10.1007/978-1-0716-0191-4_10.
C. Schirmer, J. Müller, N. Steffen, S. Werner, R. Eibl-Schindler, and D. Eibl, “How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale,” in Animal cell biotechnology : methods and protocols, R. Pörtner, Ed. New York: Springer, 2020, pp. 169–186. doi: 10.1007/978-1-0716-0191-4_10.
SCHIRMER, Cedric, Jan MÜLLER, Nina STEFFEN, Sören WERNER, Regine EIBL-SCHINDLER und Dieter EIBL, 2020. How to produce mAbs in a cube-shaped stirred single-use bioreactor at 200 L scale. In: Ralf PÖRTNER (Hrsg.), Animal cell biotechnology : methods and protocols. New York: Springer. S. 169–186. ISBN 978-1-0716-0190-7
Schirmer, Cedric, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl-Schindler, and Dieter Eibl. 2020. “How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.” In Animal Cell Biotechnology : Methods and Protocols, edited by Ralf Pörtner, 169–86. New York: Springer. https://doi.org/10.1007/978-1-0716-0191-4_10.
Schirmer, Cedric, et al. “How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.” Animal Cell Biotechnology : Methods and Protocols, edited by Ralf Pörtner, Springer, 2020, pp. 169–86, https://doi.org/10.1007/978-1-0716-0191-4_10.
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