Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale  L.) roots

Trifan, Adriana; Opitz, Sebastian; Josuran, Roland; Grubelnik, Andreas; Esslinger, Nils; Peter, Samuel; Bräm, Sarah; Meier, Nadja; Wolfram, Evelyn

doi:10.1016/j.fct.2017.12.051

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots
Authors:	Trifan, Adriana Opitz, Sebastian Josuran, Roland Grubelnik, Andreas Esslinger, Nils Peter, Samuel Bräm, Sarah Meier, Nadja Wolfram, Evelyn
DOI:	10.1016/j.fct.2017.12.051
Published in:	Food and Chemical Toxicology
Volume(Issue):	112
Page(s):	178
Pages to:	187
Issue Date:	27-Dec-2017
Publisher / Ed. Institution:	Elsevier
ISSN:	0278-6915 1873-6351
Language:	English
Subjects:	Antioxidant activity; Phenolic compound; Pyrrolizidine alkaloid; Salvianolic acid; Symphytum officinale L.
Subject (DDC):	572: Biochemistry 580: Plants (Botany) 615: Pharmacology and therapeutics
Abstract:	Comfrey root preparations are used for the external treatment of joint distortions and myalgia, due to its analgesic and anti-inflammatory properties. Up to date, key activity-determining constituents of comfrey root extracts have not been completely elucidated. Therefore, we applied different approaches to further characterize a comfrey root extract (65% ethanol). The phenolic profile of comfrey root sample was characterized by HPLC-DAD-QTOF-MS/MS. Rosmarinic acid was identified as main phenolic constituent (7.55 mg/g extract). Moreover, trimers and tetramers of caffeic acid (isomers of salvianolic acid A, B and C) were identified and quantified for the first time in comfrey root. In addition, pyrrolizidine alkaloids were evaluated by HPLC-QQQ-MS/MS and acetylintermedine, acetyllycopsamine and their N-oxides were determined as major pyrrolizidine alkaloids in the comfrey root sample. Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. Comfrey root extract exhibited significant antioxidant activities when compared to known antioxidants. Thus, comfrey root is an important source of phenolic compounds endowed with antioxidant activity which may contribute to the overall bioactivity of Symphytum preparations.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1958
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Trifan, A., Opitz, S., Josuran, R., Grubelnik, A., Esslinger, N., Peter, S., Bräm, S., Meier, N., & Wolfram, E. (2017). Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots. Food and Chemical Toxicology, 112, 178–187. https://doi.org/10.1016/j.fct.2017.12.051

Trifan, A. et al. (2017) ‘Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots’, Food and Chemical Toxicology, 112, pp. 178–187. Available at: https://doi.org/10.1016/j.fct.2017.12.051.

A. Trifan et al., “Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots,” Food and Chemical Toxicology, vol. 112, pp. 178–187, Dec. 2017, doi: 10.1016/j.fct.2017.12.051.

TRIFAN, Adriana, Sebastian OPITZ, Roland JOSURAN, Andreas GRUBELNIK, Nils ESSLINGER, Samuel PETER, Sarah BRÄM, Nadja MEIER und Evelyn WOLFRAM, 2017. Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots. Food and Chemical Toxicology. 27 Dezember 2017. Bd. 112, S. 178–187. DOI 10.1016/j.fct.2017.12.051

Trifan, Adriana, Sebastian Opitz, Roland Josuran, Andreas Grubelnik, Nils Esslinger, Samuel Peter, Sarah Bräm, Nadja Meier, and Evelyn Wolfram. 2017. “Is Comfrey Root More than Toxic Pyrrolizidine Alkaloids? Salvianolic Acids among Antioxidant Polyphenols in Comfrey (Symphytum Officinale L.) Roots.” Food and Chemical Toxicology 112 (December): 178–87. https://doi.org/10.1016/j.fct.2017.12.051.

Trifan, Adriana, et al. “Is Comfrey Root More than Toxic Pyrrolizidine Alkaloids? Salvianolic Acids among Antioxidant Polyphenols in Comfrey (Symphytum Officinale L.) Roots.” Food and Chemical Toxicology, vol. 112, Dec. 2017, pp. 178–87, https://doi.org/10.1016/j.fct.2017.12.051.