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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro
Authors: Nytko, Katarzina J.
Thumser-Henner, Pauline
Weyland, Mathias S.
Scheidegger, Stephan
Rohrer Bley, Carla
et. al: No
DOI: 10.1371/journal.pone.0216744
Published in: PLOS ONE
Volume(Issue): 14
Issue: 5
Page(s): e0216744
Issue Date: 2019
Publisher / Ed. Institution: Public Library of Science
ISSN: 1932-6203
Language: English
Subjects: Hyperthermia; Radiation therapy
Subject (DDC): 615: Pharmacology and therapeutics
Abstract: Objective Aims were to investigate sensitivity of various human and canine cancer cell lines to hyperthermia and the influence of particular treatment conditions, and to analyze the DNA-damage response and mode of cell death in cell line radiosensitized by hyperthermia. Additionally, we were interested in the involvement of HSP70 in radiosensitization. Methods Radiosensitization by hyperthermia was determined in a panel of human and canine cancer cell lines using clonogenic cell survival assay, as well as levels of heat shock proteins (HSPs) using immunoblotting. The influence of the hyperthermia-radiotherapy time gap, different temperatures and the order of treatments on clonogenicity of hyperthermia-sensitive A549 cells was investigated. Additionally, DNA damage and cell death were assessed by Comet assay and an apoptosis/necrosis assay. Further we induced transient knockdown in A549 cells to test HSP70’s involvement in radiosensitization. Results Out of eight cell lines tested, only two (A549 and Abrams) showed significant decrease in clonogenic cell survival when pre-treated with hyperthermia at 42˚C. Strong induction of HSP70 upon thermoradiotherapy (HT-RT) treatment was found in all cell lines. Transient knockdown of HSP70 in A549 cells did not result in decrease of clonogenic cell survival in response to HT-RT. Conclusion Tumor cell-type, temperature and order of treatment play an important role in radiosensitization by hyperthermia. However, hyperthermia has limited potency to radiosensitize canine cancer cells grown in a 2D cell culture setting presented here. DNA damage and apoptosis/necrosis did not increase upon combined treatment and cytosolic levels of HSP70 appear not to play critical role in the radiosensitization of A549 cells.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: School of Engineering
Organisational Unit: Institute of Applied Mathematics and Physics (IAMP)
Published as part of the ZHAW project: Dynamic Thermal Dosimetric Concept for Evaluating Synergistic Effect of Combined Hyperthermia-Radiotherapy (RT-HT)
Appears in collections:Publikationen School of Engineering

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