Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD

Schenk, A; Ziegler, LN; Meier, Nadja; Peter, Samuel; Wolfram, Evelyn

doi:10.1055/s-0037-1608215

Publication type:	Conference poster
Type of review:	Peer review (abstract)
Title:	Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD
Authors:	Schenk, A Ziegler, LN Meier, Nadja Peter, Samuel Wolfram, Evelyn
DOI:	10.1055/s-0037-1608215
Published in:	Planta Medica International Open
Volume(Issue):	4
Conference details:	65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017), Basel, 3-6 September 2017
Issue Date:	24-Oct-2017
Publisher / Ed. Institution:	Thieme
ISSN:	2509-6656
Language:	English
Subject (DDC):	572: Biochemistry 615: Pharmacology and therapeutics 660.6: Biotechnology
Abstract:	Using a coreshell column, the new UHPLC-DAD method allows almost a baseline separation of seven sennosides and the monomeric hydroxyanthracene glycosides rhein 8-glycoside and aloe emodin 8-glycoside in senna pod dry extract and in a senna-fig syrup preparation. UV and mass spectrometry studies show that none of the peaks of interest is underlaid by flavonoids, a fact which, in the case of other LC methods for sennosides, requires a sample processing by means of SPE. Sennosides are determined by sennoside B, monomeric hydroyanthracene glycosides by rhein 8-glycoside as external standard. The combined determination of all hyrdoxyanthracene glycosides is important for pharmaceutical stability testing because sennosides are partly degraded to rhein- and aloe emodin 8-glycoside. In the posterior part of the chromatogram the aglycones rhein and aloe emodine can be determined. The complete assay runs 19 min.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1964
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Schenk, A., Ziegler, L., Meier, N., Peter, S., & Wolfram, E. (2017). Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD [Conference poster]. Planta Medica International Open, 4. https://doi.org/10.1055/s-0037-1608215

Schenk, A. et al. (2017) ‘Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD’, in Planta Medica International Open. Thieme. Available at: https://doi.org/10.1055/s-0037-1608215.

A. Schenk, L. Ziegler, N. Meier, S. Peter, and E. Wolfram, “Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD,” in Planta Medica International Open, Oct. 2017, vol. 4. doi: 10.1055/s-0037-1608215.

SCHENK, A, LN ZIEGLER, Nadja MEIER, Samuel PETER und Evelyn WOLFRAM, 2017. Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD. In: Planta Medica International Open. Conference poster. Thieme. 24 Oktober 2017

Schenk, A, LN Ziegler, Nadja Meier, Samuel Peter, and Evelyn Wolfram. 2017. “Combined Determination of Sennosides, Monomeric Hydroxyanthacene Glycosides and Anthraquinone Aglycones by UHPLC-DAD.” Conference poster. In Planta Medica International Open. Vol. 4. Thieme. https://doi.org/10.1055/s-0037-1608215.

Schenk, A., et al. “Combined Determination of Sennosides, Monomeric Hydroxyanthacene Glycosides and Anthraquinone Aglycones by UHPLC-DAD.” Planta Medica International Open, vol. 4, Thieme, 2017, https://doi.org/10.1055/s-0037-1608215.