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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-20335
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dc.contributor.authorArn, Fabienne-
dc.contributor.authorFrasson, David-
dc.contributor.authorKroslakova, Ivana-
dc.contributor.authorRezzonico, Fabio-
dc.contributor.authorPothier, Joël-
dc.contributor.authorRiedl, Rainer-
dc.contributor.authorSievers, Martin-
dc.date.accessioned2020-08-10T09:28:00Z-
dc.date.available2020-08-10T09:28:00Z-
dc.date.issued2020-
dc.identifier.issn0009-4293de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/20335-
dc.description.abstractActinomycetes strains isolated from different habitats in Switzerland were investigated for production of antibacterial and antitumoral compounds. Based on partial 16S rRNA gene sequences, the isolated strains were identified to genus level. Streptomyces as the largest genus of Actinobacteriawas isolated the most frequently. A screening assay using the OmniLog instrument was established to facilitate the detection of active compounds from actinomycetes. Extracts prepared from the cultivated strains able to inhibit Staphylococcus aureusand Escherichia coliwere further analysed by HPLC and MALDI-TOF MS to identify the produced antibiotics. In this study, the bioactive compound echinomycin was identified from two isolated Streptomycesstrains. Natural compounds similar to TPU-0037-C, azalomycin F4a 2-ethylpentyl ester, a derivative of bafilomycin A1, milbemycin-α8 and dihydropicromycin were detected from different isolated Streptomyces strains. Milbemycin-α8 showed cytotoxic activity against HT-29 colon cancer cells. The rare actinomycete,Micromonospora sp. Stup16_C148 produced a compound that matches with the antibiotic bottromycin A2. The draft genome sequence from Actinokineospora strain B136.1 was determined using Illumina and nanopore-based technologies. The isolated strain was not able to produce antibacterial compounds under standard cultivation conditions. The antiSMASH bioinformatics analyses of the genome from strain B136.1 identified biosynthetic gene clusters with identity values between 4% to 90% to known gene clusters encoding antibiotics. The combinations of cultivation conditions, screening assays, analytical methods and genome mining are important tools to characterize strains of actinomycetes for the identification of their potential to produce natural compounds with antimicrobial activity.de_CH
dc.language.isoende_CH
dc.publisherSchweizerische Chemische Gesellschaftde_CH
dc.relation.ispartofChimiade_CH
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/de_CH
dc.subjectRNAde_CH
dc.subjectStreptomycesde_CH
dc.subjectSwitzerlandde_CH
dc.subjectActinobacteriade_CH
dc.subjectRibosomalde_CH
dc.subject16Sde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleIsolation and identification of actinomycetes strains from Switzerland and their biotechnological potentialde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.2533/chimia.2020.382de_CH
dc.identifier.doi10.21256/zhaw-20335-
dc.identifier.pmid32482215de_CH
zhaw.funding.euNode_CH
zhaw.issue5de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end390de_CH
zhaw.pages.start382de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume74de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMikrobiologiede_CH
zhaw.webfeedHigh Performance Computing (HPC)de_CH
zhaw.webfeedCC Drug Discoveryde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Arn, F., Frasson, D., Kroslakova, I., Rezzonico, F., Pothier, J., Riedl, R., & Sievers, M. (2020). Isolation and identification of actinomycetes strains from Switzerland and their biotechnological potential. Chimia, 74(5), 382–390. https://doi.org/10.2533/chimia.2020.382
Arn, F. et al. (2020) ‘Isolation and identification of actinomycetes strains from Switzerland and their biotechnological potential’, Chimia, 74(5), pp. 382–390. Available at: https://doi.org/10.2533/chimia.2020.382.
F. Arn et al., “Isolation and identification of actinomycetes strains from Switzerland and their biotechnological potential,” Chimia, vol. 74, no. 5, pp. 382–390, 2020, doi: 10.2533/chimia.2020.382.
ARN, Fabienne, David FRASSON, Ivana KROSLAKOVA, Fabio REZZONICO, Joël POTHIER, Rainer RIEDL und Martin SIEVERS, 2020. Isolation and identification of actinomycetes strains from Switzerland and their biotechnological potential. Chimia. 2020. Bd. 74, Nr. 5, S. 382–390. DOI 10.2533/chimia.2020.382
Arn, Fabienne, David Frasson, Ivana Kroslakova, Fabio Rezzonico, Joël Pothier, Rainer Riedl, and Martin Sievers. 2020. “Isolation and Identification of Actinomycetes Strains from Switzerland and Their Biotechnological Potential.” Chimia 74 (5): 382–90. https://doi.org/10.2533/chimia.2020.382.
Arn, Fabienne, et al. “Isolation and Identification of Actinomycetes Strains from Switzerland and Their Biotechnological Potential.” Chimia, vol. 74, no. 5, 2020, pp. 382–90, https://doi.org/10.2533/chimia.2020.382.


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