Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut

Martins, Leonor; Fernandes, Camila; Blom, Jochen; Dia, Nay C.; Pothier, Joël F.; Tavares, Fernando

doi:10.1099/ijsem.0.004386

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-20508

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut
Authors:	Martins, Leonor Fernandes, Camila Blom, Jochen Dia, Nay C. Pothier, Joël F. Tavares, Fernando
et. al:	No
DOI:	10.1099/ijsem.0.004386 10.21256/zhaw-20508
Published in:	International Journal of Systematic and Evolutionary Microbiology
Volume(Issue):	70
Issue:	12
Page(s):	6024
Pages to:	6031
Issue Date:	14-Sep-2020
Publisher / Ed. Institution:	Microbiology Society
ISSN:	1466-5026 1466-5034
Language:	English
Subjects:	Xanthomonas; New species; Pathogenicity; Walnut
Subject (DDC):	570: Biology
Abstract:	We describe a novel species isolated from walnut (Juglans regia) which comprises non-pathogenic and pathogenic strains on walnut. The isolates, obtained from a single ornamental walnut tree showing disease symptoms, grew on yeast extract-dextrose-carbonate agar as mucoid yellow colonies characteristic of Xanthomonas species. Pathogenicity assays showed that while strain CPBF 424T causes disease in walnut, strain CPBF 367 was non-pathogenic on walnut leaves. Biolog GEN III metabolic profiles disclosed some differences between strains CPBF 367 and CPBF 424T and other xanthomonads. Multilocus sequence analysis with seven housekeeping genes (fyuA, gyrB, rpoD, atpD, dnaK, efp, glnA) grouped these strains in a distinct cluster from Xanthomonas arboricola pv. juglandis and closer to Xanthomonas prunicola and Xanthomonas arboricola pv. populi. Average nucleotide identity (ANI) analysis results displayed similarity values below 93 % to X. arboricola strains. Meanwhile ANI and digital DNA-DNA hybridization similarity values were below 89 and 50 % to non-arboricola Xanthomonas strains, respectively, revealing that they do not belong to any previously described Xanthomonas species. Furthermore, the two strains show over 98 % similarity to each other. Genomic analysis shows that strain CPBF 424T harbours a complete type III secretion system and several type III effector proteins, in contrast with strain CPBF 367, shown to be non-pathogenic in plant bioassays. Taking these data altogether, we propose that strains CPBF 367 and CPBF 424T belong to a new species herein named Xanthomonas euroxanthea sp. nov., with CPBF 424T (=LMG 31037T=CCOS 1891T=NCPPB 4675T) as the type strain.
URI:	https://digitalcollection.zhaw.ch/handle/11475/20508
Fulltext version:	Published version
License (according to publishing contract):	CC BY-NC 4.0: Attribution - Non commercial 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Xhortomics: Entwicklung von diagnostischen und epidemiologischen Hilfsmitteln für die Xanthomonas hortorum Spezies Gruppe basierend auf OMICs Technologien
Appears in collections:	Publikationen Life Sciences und Facility Management

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Martins, L., Fernandes, C., Blom, J., Dia, N. C., Pothier, J. F., & Tavares, F. (2020). Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut. International Journal of Systematic and Evolutionary Microbiology, 70(12), 6024–6031. https://doi.org/10.1099/ijsem.0.004386

Martins, L. et al. (2020) ‘Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut’, International Journal of Systematic and Evolutionary Microbiology, 70(12), pp. 6024–6031. Available at: https://doi.org/10.1099/ijsem.0.004386.

L. Martins, C. Fernandes, J. Blom, N. C. Dia, J. F. Pothier, and F. Tavares, “Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut,” International Journal of Systematic and Evolutionary Microbiology, vol. 70, no. 12, pp. 6024–6031, Sep. 2020, doi: 10.1099/ijsem.0.004386.

MARTINS, Leonor, Camila FERNANDES, Jochen BLOM, Nay C. DIA, Joël F. POTHIER und Fernando TAVARES, 2020. Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut. International Journal of Systematic and Evolutionary Microbiology. 14 September 2020. Bd. 70, Nr. 12, S. 6024–6031. DOI 10.1099/ijsem.0.004386

Martins, Leonor, Camila Fernandes, Jochen Blom, Nay C. Dia, Joël F. Pothier, and Fernando Tavares. 2020. “Xanthomonas Euroxanthea Sp. Nov. : A New Xanthomonad Species Including Pathogenic and Non-Pathogenic Strains of Walnut.” International Journal of Systematic and Evolutionary Microbiology 70 (12): 6024–31. https://doi.org/10.1099/ijsem.0.004386.

Martins, Leonor, et al. “Xanthomonas Euroxanthea Sp. Nov. : A New Xanthomonad Species Including Pathogenic and Non-Pathogenic Strains of Walnut.” International Journal of Systematic and Evolutionary Microbiology, vol. 70, no. 12, Sept. 2020, pp. 6024–31, https://doi.org/10.1099/ijsem.0.004386.