Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites

Stefanache, Camelia Paula; Imseng, Nicole; Meier, Beat; Tanase, Catalin; Eibl-Schindler, Regine; Peter, Samuel; Wolfram, Evelyn

doi:10.1055/s-0033-1352447

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites
Authors:	Stefanache, Camelia Paula Imseng, Nicole Meier, Beat Tanase, Catalin Eibl-Schindler, Regine Peter, Samuel Wolfram, Evelyn
DOI:	10.1055/s-0033-1352447
Published in:	Planta Medica
Volume(Issue):	79
Issue:	13
Issue Date:	2013
Publisher / Ed. Institution:	Thieme
ISSN:	0032-0943
Language:	English
Subject (DDC):	660.6: Biotechnology
Abstract:	Arnica montana L. is a medicinal plant with traditional use to treat sprains, bruises, rheumatic and muscular aches based on the anti-inflammatory properties of sesquiterpene lactones (SL), mainly esters of helenalin and dihydrohelenalin. Overexploitation has led to conservation actions in the majority of European countries, involving in vitro cultivation techniques as a sustainable alternative. Cell suspension cultures of A. montana have not been reported extensively, although a sustainable biotechnological process in bioreactors for production of the active plant secondary metabolites is of great interest for industry. The starting plant material consisted of seeds, collected from wild populations in the Romanian Eastern Carpathians. Germination in aseptic environment lead to sterile plantlets for the micropropagation process. Leaf fragments of about 1 cm2 were placed on several medium variants – MS medium supplemented with different amount of 2,4-dichlorophenoxyacetic acid and benzylaminopurine for callus induction. First qualitative results show that suspension cultures from the obtained calli in small scale single-use bioreactors have been successfully established. Due to small biomass amounts, common HPLC and HPTLC methods from the Ph Eur Monograph on Arnica for SL, flavonoids and phenolic acids using HPLC and HPTLC had to be optimized to very low sample amounts. Quantitative assessment of the desired secondary metabolites is under way in parallel to the continuous culturing process. The final aim of the study is to show differences in secondary metabolite content in plant material from the field, callus and cell suspensions.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2084
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Stefanache, C. P., Imseng, N., Meier, B., Tanase, C., Eibl-Schindler, R., Peter, S., & Wolfram, E. (2013). Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites. Planta Medica, 79(13). https://doi.org/10.1055/s-0033-1352447

Stefanache, C.P. et al. (2013) ‘Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites’, Planta Medica, 79(13). Available at: https://doi.org/10.1055/s-0033-1352447.

C. P. Stefanache et al., “Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites,” Planta Medica, vol. 79, no. 13, 2013, doi: 10.1055/s-0033-1352447.

STEFANACHE, Camelia Paula, Nicole IMSENG, Beat MEIER, Catalin TANASE, Regine EIBL-SCHINDLER, Samuel PETER und Evelyn WOLFRAM, 2013. Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites. Planta Medica. 2013. Bd. 79, Nr. 13. DOI 10.1055/s-0033-1352447

Stefanache, Camelia Paula, Nicole Imseng, Beat Meier, Catalin Tanase, Regine Eibl-Schindler, Samuel Peter, and Evelyn Wolfram. 2013. “Arnica Montana L. Cell Suspension Culture as a Biotechnological Approach for the Production of Bioactive Secondary Metabolites.” Planta Medica 79 (13). https://doi.org/10.1055/s-0033-1352447.

Stefanache, Camelia Paula, et al. “Arnica Montana L. Cell Suspension Culture as a Biotechnological Approach for the Production of Bioactive Secondary Metabolites.” Planta Medica, vol. 79, no. 13, 2013, https://doi.org/10.1055/s-0033-1352447.