Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-21820
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dc.contributor.authorShrestha, Neeta-
dc.contributor.authorGall, Flavio-
dc.contributor.authorVesin, Jonathan-
dc.contributor.authorChambon, Marc-
dc.contributor.authorTurcatti, Gerardo-
dc.contributor.authorFotiadis, Dimitrios-
dc.contributor.authorRiedl, Rainer-
dc.contributor.authorPlattet, Philippe-
dc.date.accessioned2021-02-18T13:53:01Z-
dc.date.available2021-02-18T13:53:01Z-
dc.date.issued2021-01-18-
dc.identifier.issn1999-4915de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/21820-
dc.description.abstractCanine distemper virus (CDV), a close relative of the human pathogen measles virus (MeV), is an enveloped, negative sense RNA virus that belongs to the genus Morbillivirus and causes severe diseases in dogs and other carnivores. Although the vaccination is available as a preventive measure against the disease, the occasional vaccination failure highlights the importance of therapeutic alternatives such as antivirals against CDV. The morbilliviral cell entry system relies on two interacting envelope glycoproteins: the attachment (H) and fusion (F) proteins. Here, to potentially discover novel entry inhibitors targeting CDV H, F and/or the cognate receptor: signaling lymphocyte activation molecule (SLAM) proteins, we designed a quantitative cell-based fusion assay that matched high-throughput screening (HTS) settings. By screening two libraries of small molecule compounds, we successfully identified two membrane fusion inhibitors (F2736-3056 and F2261-0043). Although both inhibitors exhibited similarities in structure and potency with the small molecule compound 3G (an AS-48 class morbilliviral F-protein inhibitor), F2736-3056 displayed improved efficacy in blocking fusion activity when a 3G-escape variant was employed. Altogether, we present a cell-based fusion assay that can be utilized not only to discover antiviral agents against CDV but also to dissect the mechanism of morbilliviral-mediated cell-binding and cell-to-cell fusion activity.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofVirusesde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectCDVde_CH
dc.subjectCell-based fusion assayde_CH
dc.subjectEnvelope glycoproteinsde_CH
dc.subjectHost cell receptorde_CH
dc.subjectInhibitor discoveryde_CH
dc.subject.ddc579: Mikrobiologiede_CH
dc.titleAntiviral screen against canine distemper virus-induced membrane fusion activityde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/v13010128de_CH
dc.identifier.doi10.21256/zhaw-21820-
dc.identifier.pmid33477492de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start128de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume13de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf183481de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management



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