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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells
Authors: Panella, Stefano
Muoio, Francesco
Jossen, Valentin
Harder, Yves
Eibl-Schindler, Regine
Tallone, Tiziano
et. al: No
DOI: 10.3390/cells10020466
Published in: Cells
Volume(Issue): 10
Issue: 2
Page(s): 466
Issue Date: 2021
Publisher / Ed. Institution: MDPI
ISSN: 2073-4409
Language: English
Subjects: UrSuppe; Defined cell culture; Xeno- and serum-free cell culture; Adipogenic differentiated adipose-derived stromal cell; White and beige/brown adipocyte; Platelet lysate
Subject (DDC): 610.28: Biomedicine, biomedical engineering
Abstract: Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell drug” that is safe, reproducible, and cost-effective. Thus, we developed our own basal defined xeno- and serum-free cell culture medium (UrSuppe), specifically formulated to grow human adipose stem cells (hASCs). With this medium, we can directly culture the stromal vascular fraction (SVF) cells in defined cell culture conditions to obtain hASCs. Cells proliferate while remaining undifferentiated, as shown by Flow Cytometry (FACS), Quantitative Reverse Transcription PCR (RT-qPCR) assays, and their secretion products. Using the UrSuppe cell culture medium, maximum cell densities between 0.51 and 0.80 × 105 cells/cm2 (=2.55–4.00 × 105 cells/mL) were obtained. As the expansion of hASCs represents only the first step in a cell therapeutic protocol or further basic research studies, we formulated two chemically defined media to differentiate the expanded hASCs in white or beige/brown adipocytes. These new media could help translate research projects into the clinical application of hASCs and study ex vivo the biology in healthy and dysfunctional states of adipocytes and their precursors. Following the cell culture system developers’ practice and obvious reasons related to the formulas’ patentability, the defined media’s composition will not be disclosed in this study.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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