Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-21921
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dc.contributor.authorPanella, Stefano-
dc.contributor.authorMuoio, Francesco-
dc.contributor.authorJossen, Valentin-
dc.contributor.authorHarder, Yves-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorTallone, Tiziano-
dc.date.accessioned2021-03-04T10:26:25Z-
dc.date.available2021-03-04T10:26:25Z-
dc.date.issued2021-
dc.identifier.issn2073-4409de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/21921-
dc.description.abstractAdipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell drug” that is safe, reproducible, and cost-effective. Thus, we developed our own basal defined xeno- and serum-free cell culture medium (UrSuppe), specifically formulated to grow human adipose stem cells (hASCs). With this medium, we can directly culture the stromal vascular fraction (SVF) cells in defined cell culture conditions to obtain hASCs. Cells proliferate while remaining undifferentiated, as shown by Flow Cytometry (FACS), Quantitative Reverse Transcription PCR (RT-qPCR) assays, and their secretion products. Using the UrSuppe cell culture medium, maximum cell densities between 0.51 and 0.80 × 105 cells/cm2 (=2.55–4.00 × 105 cells/mL) were obtained. As the expansion of hASCs represents only the first step in a cell therapeutic protocol or further basic research studies, we formulated two chemically defined media to differentiate the expanded hASCs in white or beige/brown adipocytes. These new media could help translate research projects into the clinical application of hASCs and study ex vivo the biology in healthy and dysfunctional states of adipocytes and their precursors. Following the cell culture system developers’ practice and obvious reasons related to the formulas’ patentability, the defined media’s composition will not be disclosed in this study.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofCellsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectUrSuppede_CH
dc.subjectDefined cell culturede_CH
dc.subjectXeno- and serum-free cell culturede_CH
dc.subjectAdipogenic differentiated adipose-derived stromal cellde_CH
dc.subjectWhite and beige/brown adipocytede_CH
dc.subjectPlatelet lysatede_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleChemically defined xeno- and serum-free cell culture medium to grow human adipose stem cellsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/cells10020466de_CH
dc.identifier.doi10.21256/zhaw-21921-
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start466de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume10de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Panella, S., Muoio, F., Jossen, V., Harder, Y., Eibl-Schindler, R., & Tallone, T. (2021). Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells. Cells, 10(2), 466. https://doi.org/10.3390/cells10020466
Panella, S. et al. (2021) ‘Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells’, Cells, 10(2), p. 466. Available at: https://doi.org/10.3390/cells10020466.
S. Panella, F. Muoio, V. Jossen, Y. Harder, R. Eibl-Schindler, and T. Tallone, “Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells,” Cells, vol. 10, no. 2, p. 466, 2021, doi: 10.3390/cells10020466.
PANELLA, Stefano, Francesco MUOIO, Valentin JOSSEN, Yves HARDER, Regine EIBL-SCHINDLER und Tiziano TALLONE, 2021. Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells. Cells. 2021. Bd. 10, Nr. 2, S. 466. DOI 10.3390/cells10020466
Panella, Stefano, Francesco Muoio, Valentin Jossen, Yves Harder, Regine Eibl-Schindler, and Tiziano Tallone. 2021. “Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.” Cells 10 (2): 466. https://doi.org/10.3390/cells10020466.
Panella, Stefano, et al. “Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.” Cells, vol. 10, no. 2, 2021, p. 466, https://doi.org/10.3390/cells10020466.


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