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dc.contributor.authorHendrawan, Siufui-
dc.contributor.authorBono, Epifania-
dc.contributor.authorHutter, Albert-
dc.contributor.authorWeber, Ursula-
dc.contributor.authorLheman, Jennifer-
dc.contributor.authorBaer, Hans U.-
dc.date.accessioned2021-03-12T14:53:48Z-
dc.date.available2021-03-12T14:53:48Z-
dc.date.issued2020-10-16-
dc.identifier.issn1552-4973de_CH
dc.identifier.issn1552-4981de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/21982-
dc.description.abstractOrthotopic liver transplantation is presently the most effectual method for the treatment of end-stage liver diseases. Though, one major issue is the restricted number of donor organs that are accessible. Hence, liver tissue engineering is under investigation with the goal of restoring liver functions. In this study, we investigated 3D porous scaffolds made of PLLA coated with a nano thick collagen layer (matrices). Primary rat dermal fibroblasts were used in a first study phase to check matrices' cytocompatibility. More than 70% of seeded cells could adhere and remain viable 24 and 48 hours after the seeding. To test the suitability of the matrices for human primary hepatocytes, HepaRG cells were seeded and analyzed for viability, adhesion rate, and functionality such as albumin secretion. About 80% of seeded HepaRG adhered to the scaffolds remaining viable up to 72 hours. Cells were homogeneously distributed in the entire scaffold with albumin secretion increasing with time. Our results indicate that PLLA collagen-coated matrices allow hepatocytes attachment and distribution throughout the 3D structure, as well as support cell functionality. Such matrices have been applied in our clinical phase II trial. Functional hepatocytes were successfully implanted in patients suffering from liver-cirrhosis with higher cell numbers and adhesions rate compared to our previous trial with the first matrix type and a general improvement in clinical condition.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofJournal of Biomedical Materials Research Part B : Applied Biomaterialsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject3D PLLA scaffoldsde_CH
dc.subjectHepatocytede_CH
dc.subjectNano-thick collagen coatingde_CH
dc.subjectTissue engineeringde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleEvaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1002/jbm.b.34738de_CH
dc.identifier.pmid33063448de_CH
zhaw.funding.euNode_CH
zhaw.issue5de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end732de_CH
zhaw.pages.start723de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume109de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeed3D Gewebe und Biofabrikationde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Hendrawan, S., Bono, E., Hutter, A., Weber, U., Lheman, J., & Baer, H. U. (2020). Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes. Journal of Biomedical Materials Research Part B : Applied Biomaterials, 109(5), 723–732. https://doi.org/10.1002/jbm.b.34738
Hendrawan, S. et al. (2020) ‘Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes’, Journal of Biomedical Materials Research Part B : Applied Biomaterials, 109(5), pp. 723–732. Available at: https://doi.org/10.1002/jbm.b.34738.
S. Hendrawan, E. Bono, A. Hutter, U. Weber, J. Lheman, and H. U. Baer, “Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes,” Journal of Biomedical Materials Research Part B : Applied Biomaterials, vol. 109, no. 5, pp. 723–732, Oct. 2020, doi: 10.1002/jbm.b.34738.
HENDRAWAN, Siufui, Epifania BONO, Albert HUTTER, Ursula WEBER, Jennifer LHEMAN und Hans U. BAER, 2020. Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes. Journal of Biomedical Materials Research Part B : Applied Biomaterials. 16 Oktober 2020. Bd. 109, Nr. 5, S. 723–732. DOI 10.1002/jbm.b.34738
Hendrawan, Siufui, Epifania Bono, Albert Hutter, Ursula Weber, Jennifer Lheman, and Hans U. Baer. 2020. “Evaluation of 3D PLLA Scaffolds Coated with Nano‐Thick Collagen as Carrier for Hepatocytes.” Journal of Biomedical Materials Research Part B : Applied Biomaterials 109 (5): 723–32. https://doi.org/10.1002/jbm.b.34738.
Hendrawan, Siufui, et al. “Evaluation of 3D PLLA Scaffolds Coated with Nano‐Thick Collagen as Carrier for Hepatocytes.” Journal of Biomedical Materials Research Part B : Applied Biomaterials, vol. 109, no. 5, Oct. 2020, pp. 723–32, https://doi.org/10.1002/jbm.b.34738.


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