Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree

Fernandes, Camila; Martins, Leonor; Teixeira, Miguel; Blom, Jochen; Pothier, Joël; Fonseca, Nuno A.; Tavares, Fernando

doi:10.3390/microorganisms9030624

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-22169

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree
Authors:	Fernandes, Camila Martins, Leonor Teixeira, Miguel Blom, Jochen Pothier, Joël Fonseca, Nuno A. Tavares, Fernando
et. al:	No
DOI:	10.3390/microorganisms9030624 10.21256/zhaw-22169
Published in:	Microorganisms
Volume(Issue):	9
Issue:	3
Page(s):	624
Issue Date:	17-Mar-2021
Publisher / Ed. Institution:	MDPI
ISSN:	2076-2607
Language:	English
Subjects:	Walnut bacterial blight; Comparative genomics; Xanthomonas arboricola pv. juglandis; Xanthomonas euroxanthea; Pathogenicity
Subject (DDC):	572: Biochemistry 579: Microbiology
Abstract:	The recent report of distinct Xanthomonas lineages of Xanthomonas arboricola pv. juglandis and Xanthomonas euroxanthea within the same walnut tree revealed that this consortium of walnut-associated Xanthomonas includes both pathogenic and nonpathogenic strains. As the implications of this co-colonization are still poorly understood, in order to unveil niche-specific adaptations, the genomes of three X. euroxanthea strains (CPBF 367, CPBF 424T, and CPBF 426) and of an X. arboricola pv. juglandis strain (CPBF 427) isolated from a single walnut tree in Loures (Portugal) were sequenced with two different technologies, Illumina and Nanopore, to provide consistent single scaffold chromosomal sequences. General genomic features showed that CPBF 427 has a genome similar to other X. arboricola pv. juglandis strains, regarding its size, number, and content of CDSs, while X. euroxanthea strains show a reduction regarding these features comparatively to X. arboricola pv. juglandis strains. Whole genome comparisons revealed remarkable genomic differences between X. arboricola pv. juglandis and X. euroxanthea strains, which translates into different pathogenicity and virulence features, namely regarding type 3 secretion system and its effectors and other secretory systems, chemotaxis-related proteins, and extracellular enzymes. Altogether, the distinct genomic repertoire of X. euroxanthea may be particularly useful to address pathogenicity emergence and evolution in walnut-associated Xanthomonas.
URI:	https://digitalcollection.zhaw.ch/handle/11475/22169
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Xhortomics: Entwicklung von diagnostischen und epidemiologischen Hilfsmitteln für die Xanthomonas hortorum Spezies Gruppe basierend auf OMICs Technologien Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads
Appears in collections:	Publikationen Life Sciences und Facility Management

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Supplementar material_proof.pdf	Supplementary Material	7.17 MB	Adobe PDF	View/Open
Table S2_Fernandes et al, 2021 MDPI_proof.xlsx	Supplementary Table S2	22.39 kB	Microsoft Excel XML	View/Open
Table S3_ Best BLAST hit results putative homologs_proof.tsv	Supplementary Table S3	436.04 kB	Unknown	View/Open
2021_Fernandes_Comparative_Genomics_Microorganisms.pdf	Main Text	1.47 MB	Adobe PDF	View/Open

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Fernandes, C., Martins, L., Teixeira, M., Blom, J., Pothier, J., Fonseca, N. A., & Tavares, F. (2021). Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree. Microorganisms, 9(3), 624. https://doi.org/10.3390/microorganisms9030624

Fernandes, C. et al. (2021) ‘Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree’, Microorganisms, 9(3), p. 624. Available at: https://doi.org/10.3390/microorganisms9030624.

C. Fernandes et al., “Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree,” Microorganisms, vol. 9, no. 3, p. 624, Mar. 2021, doi: 10.3390/microorganisms9030624.

FERNANDES, Camila, Leonor MARTINS, Miguel TEIXEIRA, Jochen BLOM, Joël POTHIER, Nuno A. FONSECA und Fernando TAVARES, 2021. Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree. Microorganisms. 17 März 2021. Bd. 9, Nr. 3, S. 624. DOI 10.3390/microorganisms9030624

Fernandes, Camila, Leonor Martins, Miguel Teixeira, Jochen Blom, Joël Pothier, Nuno A. Fonseca, and Fernando Tavares. 2021. “Comparative Genomics of Xanthomonas Euroxanthea and Xanthomonas Arboricola Pv. Juglandis Strains Isolated from a Single Walnut Host Tree.” Microorganisms 9 (3): 624. https://doi.org/10.3390/microorganisms9030624.

Fernandes, Camila, et al. “Comparative Genomics of Xanthomonas Euroxanthea and Xanthomonas Arboricola Pv. Juglandis Strains Isolated from a Single Walnut Host Tree.” Microorganisms, vol. 9, no. 3, Mar. 2021, p. 624, https://doi.org/10.3390/microorganisms9030624.