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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Human adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated status
Authors: Muoio, Francesco
Panella, Stefano
Jossen, Valentin
Lindner, Matias
Harder, Yves
Müller, Michele
Eibl, Regine
Tallone, Tiziano
et. al: No
DOI: 10.3390/jfb12020025
Published in: Journal of Functional Biomaterials
Volume(Issue): 12
Issue: 2
Page(s): 25
Issue Date: 2021
Publisher / Ed. Institution: MDPI
ISSN: 2079-4983
Language: English
Subjects: Serum- and xeno-free cell culture medium; UrSuppe; Biodegradable microcarrier (MC); Biomaterial; Human adipose stem cell (hASC)
Subject (DDC): 610.28: Biomedicine, biomedical engineering
660.6: Biotechnology
Abstract: Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus, they are a viable alternative to static planar cultivation systems to obtain high numbers of clinical-grade hASCs. Nevertheless, the production of considerable biomass in a short time must not be achieved to the detriment of the cells’ quality. To facilitate the scalable expansion of hASC, we have developed a new serum- and xeno-free medium (UrSuppe) and a biodegradable microcarrier (BR44). In this study, we investigated whether the culture of hASCs in defined serum-free conditions on microcarriers (3D) or on planar (2D) cell culture vessels may influence the expression of some marker genes linked with the immature degree or the differentiated status of the cells. Furthermore, we investigated whether the biomaterials, which form our biodegradable MCs, may affect cell behavior and differentiation. The results confirmed that the quality and the undifferentiated status of the hASCs are very well preserved when they grow on BR44 MCs in defined serum-free conditions. Indeed, the ASCs showed a gene expression profile more compatible with an undifferentiated status than the same cells grown under standard planar conditions.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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