Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-22322
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dc.contributor.authorMuoio, Francesco-
dc.contributor.authorPanella, Stefano-
dc.contributor.authorJossen, Valentin-
dc.contributor.authorLindner, Matias-
dc.contributor.authorHarder, Yves-
dc.contributor.authorMüller, Michele-
dc.contributor.authorEibl, Regine-
dc.contributor.authorTallone, Tiziano-
dc.date.accessioned2021-04-22T09:07:32Z-
dc.date.available2021-04-22T09:07:32Z-
dc.date.issued2021-
dc.identifier.issn2079-4983de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/22322-
dc.description.abstractHuman adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus, they are a viable alternative to static planar cultivation systems to obtain high numbers of clinical-grade hASCs. Nevertheless, the production of considerable biomass in a short time must not be achieved to the detriment of the cells’ quality. To facilitate the scalable expansion of hASC, we have developed a new serum- and xeno-free medium (UrSuppe) and a biodegradable microcarrier (BR44). In this study, we investigated whether the culture of hASCs in defined serum-free conditions on microcarriers (3D) or on planar (2D) cell culture vessels may influence the expression of some marker genes linked with the immature degree or the differentiated status of the cells. Furthermore, we investigated whether the biomaterials, which form our biodegradable MCs, may affect cell behavior and differentiation. The results confirmed that the quality and the undifferentiated status of the hASCs are very well preserved when they grow on BR44 MCs in defined serum-free conditions. Indeed, the ASCs showed a gene expression profile more compatible with an undifferentiated status than the same cells grown under standard planar conditions.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofJournal of Functional Biomaterialsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectSerum- and xeno-free cell culture mediumde_CH
dc.subjectUrSuppede_CH
dc.subjectBiodegradable microcarrier (MC)de_CH
dc.subjectBiomaterialde_CH
dc.subjectHuman adipose stem cell (hASC)de_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleHuman adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated statusde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/jfb12020025de_CH
dc.identifier.doi10.21256/zhaw-22322-
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start25de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Muoio, F., Panella, S., Jossen, V., Lindner, M., Harder, Y., Müller, M., Eibl, R., & Tallone, T. (2021). Human adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated status. Journal of Functional Biomaterials, 12(2), 25. https://doi.org/10.3390/jfb12020025
Muoio, F. et al. (2021) ‘Human adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated status’, Journal of Functional Biomaterials, 12(2), p. 25. Available at: https://doi.org/10.3390/jfb12020025.
F. Muoio et al., “Human adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated status,” Journal of Functional Biomaterials, vol. 12, no. 2, p. 25, 2021, doi: 10.3390/jfb12020025.
MUOIO, Francesco, Stefano PANELLA, Valentin JOSSEN, Matias LINDNER, Yves HARDER, Michele MÜLLER, Regine EIBL und Tiziano TALLONE, 2021. Human adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated status. Journal of Functional Biomaterials. 2021. Bd. 12, Nr. 2, S. 25. DOI 10.3390/jfb12020025
Muoio, Francesco, Stefano Panella, Valentin Jossen, Matias Lindner, Yves Harder, Michele Müller, Regine Eibl, and Tiziano Tallone. 2021. “Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status.” Journal of Functional Biomaterials 12 (2): 25. https://doi.org/10.3390/jfb12020025.
Muoio, Francesco, et al. “Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status.” Journal of Functional Biomaterials, vol. 12, no. 2, 2021, p. 25, https://doi.org/10.3390/jfb12020025.


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