Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps

Blaschczok, Katharina; Kaiser, Stephan C.; Löffelholz, Christian; Imseng, Nicole; Burkart, Jürg; Bösch, Pascal; Dornfeld, Wolfgang; Eibl-Schindler, Regine; Eibl, Dieter

doi:10.1002/cite.201200135

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps
Authors:	Blaschczok, Katharina Kaiser, Stephan C. Löffelholz, Christian Imseng, Nicole Burkart, Jürg Bösch, Pascal Dornfeld, Wolfgang Eibl-Schindler, Regine Eibl, Dieter
DOI:	10.1002/cite.201200135
Published in:	Chemie Ingenieur Technik
Volume(Issue):	85
Issue:	1-2
Page(s):	144
Pages to:	152
Issue Date:	Feb-2013
Publisher / Ed. Institution:	Wiley
Publisher / Ed. Institution:	Weinheim
ISSN:	0009-286X 1522-2640
Language:	English
Subject (DDC):	620: Engineering
Abstract:	Mechanical stress caused to transfected Chinese hamster ovary (CHO) suspension cells by reusable and single-use magnetically levitated, bearingless centrifugal pumps was investigated. Cell death rates were determined for different pump speeds and compared with data from a peristaltic and a 4-piston diaphragm pump. Furthermore, the fluid distribution inside the PuraLev® 200 pump was modeled using computational fluid dynamics. The results reveal considerably lower mechanical stress to CHO cells caused by the magnetically levitated bearingless centrifugal pumps than by the peristaltic and diaphragm pump.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2242
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Blaschczok, K., Kaiser, S. C., Löffelholz, C., Imseng, N., Burkart, J., Bösch, P., Dornfeld, W., Eibl-Schindler, R., & Eibl, D. (2013). Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps. Chemie Ingenieur Technik, 85(1-2), 144–152. https://doi.org/10.1002/cite.201200135

Blaschczok, K. et al. (2013) ‘Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps’, Chemie Ingenieur Technik, 85(1-2), pp. 144–152. Available at: https://doi.org/10.1002/cite.201200135.

K. Blaschczok et al., “Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps,” Chemie Ingenieur Technik, vol. 85, no. 1-2, pp. 144–152, Feb. 2013, doi: 10.1002/cite.201200135.

BLASCHCZOK, Katharina, Stephan C. KAISER, Christian LÖFFELHOLZ, Nicole IMSENG, Jürg BURKART, Pascal BÖSCH, Wolfgang DORNFELD, Regine EIBL-SCHINDLER und Dieter EIBL, 2013. Investigations on mechanical stress caused to CHO suspension cells by standard and single-use pumps. Chemie Ingenieur Technik. Februar 2013. Bd. 85, Nr. 1-2, S. 144–152. DOI 10.1002/cite.201200135

Blaschczok, Katharina, Stephan C. Kaiser, Christian Löffelholz, Nicole Imseng, Jürg Burkart, Pascal Bösch, Wolfgang Dornfeld, Regine Eibl-Schindler, and Dieter Eibl. 2013. “Investigations on Mechanical Stress Caused to CHO Suspension Cells by Standard and Single-Use Pumps.” Chemie Ingenieur Technik 85 (1-2): 144–52. https://doi.org/10.1002/cite.201200135.

Blaschczok, Katharina, et al. “Investigations on Mechanical Stress Caused to CHO Suspension Cells by Standard and Single-Use Pumps.” Chemie Ingenieur Technik, vol. 85, no. 1-2, Feb. 2013, pp. 144–52, https://doi.org/10.1002/cite.201200135.