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https://doi.org/10.21256/zhaw-1590| Publication type: | Article in scientific journal |
| Type of review: | Not specified |
| Title: | On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur |
| Authors: | Jossen, Valentin Schirmaier, Carmen John, Gernot T. Eibl, Dieter Eibl-Schindler, Regine |
| DOI: | 10.1007/s12268-015-0597-4 10.21256/zhaw-1590 |
| Published in: | BIOspektrum |
| Volume(Issue): | 21 |
| Issue: | 4 |
| Page(s): | 422 |
| Pages to: | 424 |
| Issue Date: | Jun-2015 |
| Publisher / Ed. Institution: | Springer |
| Publisher / Ed. Institution: | Berlin |
| ISSN: | 0947-0867 1868-6249 |
| Language: | German |
| Subject (DDC): | 570: Biology |
| Abstract: | Spinner flasks are often used for microcarrie-based cultivations of human mesenchymal stem cells (hMSCs). Normally, they are not equipped with pH and dissolved oxygen (DO) probes. This application note describes the cultivation of hMSCs in single-use spinner flasks equipped with optical pH (SP-HP8) and DO (SP-PSt3) sensors for the first time. While reaching peak cell numbers between 4.1 × 107 cells and 5.9 × 107 cells in two cultivation runs, reliable DO and pH data were delivered. |
| Further description: | Erworben im Rahmen der Schweizer Nationallizenzen (http://www.nationallizenzen.ch) |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2271 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Restricted until: | 2020-07-01 |
| Departement: | Life Sciences and Facility Management |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2015_Jossen_On-line-pH_Bio Spektrum.pdf | 502.46 kB | Adobe PDF |  View/Open |
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Jossen, V., Schirmaier, C., John, G. T., Eibl, D., & Eibl-Schindler, R. (2015). On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur. BIOspektrum, 21(4), 422–424. https://doi.org/10.1007/s12268-015-0597-4
Jossen, V. et al. (2015) ‘On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur’, BIOspektrum, 21(4), pp. 422–424. Available at: https://doi.org/10.1007/s12268-015-0597-4.
V. Jossen, C. Schirmaier, G. T. John, D. Eibl, and R. Eibl-Schindler, “On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur,” BIOspektrum, vol. 21, no. 4, pp. 422–424, Jun. 2015, doi: 10.1007/s12268-015-0597-4.
JOSSEN, Valentin, Carmen SCHIRMAIER, Gernot T. JOHN, Dieter EIBL und Regine EIBL-SCHINDLER, 2015. On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur. BIOspektrum. Juni 2015. Bd. 21, Nr. 4, S. 422–424. DOI 10.1007/s12268-015-0597-4
Jossen, Valentin, Carmen Schirmaier, Gernot T. John, Dieter Eibl, and Regine Eibl-Schindler. 2015. “On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur.” BIOspektrum 21 (4): 422–24. https://doi.org/10.1007/s12268-015-0597-4.
Jossen, Valentin, et al. “On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur.” BIOspektrum, vol. 21, no. 4, June 2015, pp. 422–24, https://doi.org/10.1007/s12268-015-0597-4.
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