Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-23358
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dc.contributor.authorMaschke, Rüdiger W.-
dc.contributor.authorSeidel, Stefan-
dc.contributor.authorBley, Thomas-
dc.contributor.authorEibl, Regine-
dc.contributor.authorEibl, Dieter-
dc.date.accessioned2021-10-30T12:35:44Z-
dc.date.available2021-10-30T12:35:44Z-
dc.date.issued2022-01-
dc.identifier.issn1369-703Xde_CH
dc.identifier.issn1873-295Xde_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/23358-
dc.description.abstractProcesses involving mammalian cell cultures - especially CHO suspension cells - dominate biopharmaceutical manufacturing. These processes are usually developed in small scale orbitally shaken cultivation systems, and thoroughly characterizing these cultivation systems is crucial to their application in research and the subsequent scale-up to production processes. With the knowledge of process engineering parameters such as oxygen transfer rate, mixing time, and power input, in combination with the demands set by the biological production system, biomass growth and product yields can be anticipated and even increased. However, the available data sources for orbitally shaken cultivation systems are often incomplete and thus not sufficient enough to generate suitable cultivation requirements. Furthermore, process engineering knowledge is inapplicable if it is not linked to the physiological demands of the cells. In the current study, a simple yet comprehensive approach for the characterization and design space prediction of orbitally shaken single-use cultivation systems is presented, including the “classical” Erlenmeyer shake flask, the cylindrical TubeSpin bioreactor and the alternately designed Optimum Growth flask. Cultivations were performed inside and outside the design space to validate the defined culture conditions, so that cultivation success (desired specific growth rates and viable cell densities) could be achieved for each cultivation system.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofBiochemical Engineering Journalde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectErlenmeyer shake flaskde_CH
dc.subjectOptimum Growth flaskde_CH
dc.subjectTubeSpin bioreactorde_CH
dc.subjectCHO suspension cell culturede_CH
dc.subjectProcess engineering characterizationde_CH
dc.subjectDesign spacede_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleDetermination of culture design spaces in shaken disposable cultivation systems for CHO suspension cell culturesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.bej.2021.108224de_CH
dc.identifier.doi10.21256/zhaw-23358-
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start108224de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume177de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Maschke, R. W., Seidel, S., Bley, T., Eibl, R., & Eibl, D. (2022). Determination of culture design spaces in shaken disposable cultivation systems for CHO suspension cell cultures. Biochemical Engineering Journal, 177, 108224. https://doi.org/10.1016/j.bej.2021.108224
Maschke, R.W. et al. (2022) ‘Determination of culture design spaces in shaken disposable cultivation systems for CHO suspension cell cultures’, Biochemical Engineering Journal, 177, p. 108224. Available at: https://doi.org/10.1016/j.bej.2021.108224.
R. W. Maschke, S. Seidel, T. Bley, R. Eibl, and D. Eibl, “Determination of culture design spaces in shaken disposable cultivation systems for CHO suspension cell cultures,” Biochemical Engineering Journal, vol. 177, p. 108224, Jan. 2022, doi: 10.1016/j.bej.2021.108224.
MASCHKE, Rüdiger W., Stefan SEIDEL, Thomas BLEY, Regine EIBL und Dieter EIBL, 2022. Determination of culture design spaces in shaken disposable cultivation systems for CHO suspension cell cultures. Biochemical Engineering Journal. Januar 2022. Bd. 177, S. 108224. DOI 10.1016/j.bej.2021.108224
Maschke, Rüdiger W., Stefan Seidel, Thomas Bley, Regine Eibl, and Dieter Eibl. 2022. “Determination of Culture Design Spaces in Shaken Disposable Cultivation Systems for CHO Suspension Cell Cultures.” Biochemical Engineering Journal 177 (January): 108224. https://doi.org/10.1016/j.bej.2021.108224.
Maschke, Rüdiger W., et al. “Determination of Culture Design Spaces in Shaken Disposable Cultivation Systems for CHO Suspension Cell Cultures.” Biochemical Engineering Journal, vol. 177, Jan. 2022, p. 108224, https://doi.org/10.1016/j.bej.2021.108224.


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