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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Clarification of yeast cell suspensions by a highly porous polyamide nanofiber sponge
Authors: Mousavi, Sara
Filipová, Lucie
Ebert, Jürgen
Heiligtag, Florian Johannes
Daumke, Ralph
Loser, Werner
Ledergerber, Bettina
Frank, Brian
Adlhart, Christian
et. al: No
DOI: 10.1016/j.seppur.2021.120273
Published in: Separation and Purification Technology
Volume(Issue): 284
Issue: 120273
Issue Date: 2022
Publisher / Ed. Institution: Elsevier
ISSN: 1383-5866
Language: English
Subjects: Yeast cell filtration; Nanofibrous polyamide sponge; Depth filtration; Pore blocking mechanism; 3D nanofiber aerogel
Subject (DDC): 660: Chemical engineering
Abstract: Depth filtration is an attractive method for initial clarification of broth for removing cells and cell debris. Electrospun nanofibers with their large specific surface area and a porous structure are known as attractive materials in filtration processes. However, dead-end filtration of cells through nanofiber mats (NFM) always leads to cake formation and increasing resistance. In this study, for the first time, a nanofiber sponge (NFS) or nanofiber aerogel was synthetized from polyamide 6 (PA6) building blocks. The NFS was flexible, highly porous and mechanically stable. The pore size of the NFS was tuned between 8 and 26 µm during the cryogenic processing step. Volumetric flux and filtration efficiency of the NFS depended on the pore size and the results were compared with those for NFM from the same PA6 nanofiber material. Dead-end filtration of Saccharomyces cerevisiae was feasible at a low differential pressure of 3.5 kPa and cell filtration efficiency was > 99 %. Modelling of the filtration process revealed that cake formation is prevented by NFS filters since cells are able to penetrate into the filter and to adsorb on their internal surface. The filtration characteristics were also compared with commercial depth filters and revealed the high flux of NFS filters along with the possibility to avoid filter aids and a low environmental impact. PA6-NFS filters may become a new and cost effective generation of filters for removing different cells or cell debris from broth and other applications.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Published as part of the ZHAW project: Nanofaserbasierte Aerogele für ein Filtrationssystem in biotechnologischen Downstream Prozessen
Appears in collections:Publikationen Life Sciences und Facility Management

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