Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH

Pulgarin, Adrian; Decker, Jérémie; Chen, Jiahua; Giannakis, Stefanos; Ludwig, Christian; Refardt, Dominik; Pick, Horst

doi:10.1016/j.watres.2022.119301

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-25997

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH
Authors:	Pulgarin, Adrian Decker, Jérémie Chen, Jiahua Giannakis, Stefanos Ludwig, Christian Refardt, Dominik Pick, Horst
et. al:	No
DOI:	10.1016/j.watres.2022.119301 10.21256/zhaw-25997
Published in:	Water Research
Volume(Issue):	226
Issue:	119301
Issue Date:	2022
Publisher / Ed. Institution:	Elsevier
ISSN:	0043-1354 1879-2448
Language:	English
Subjects:	Photo-Fenton; Near-neutral pH; Rotifers; Microalgae cultivation; Iron-citrate complex; Chlorella vulgaris; Contamination; Pest control
Subject (DDC):	570: Biology
Abstract:	In this study, a citrate-modified photo-Fenton process was successfully applied to decontaminate a Chlorella vulgaris microalgae culture spiked with the rotifer Brachionus calyciflorus (5 individuals mL−1). The applied treatment (1 mg L−1 Fe2+, 20 mg L−1 H2O2, 17.5 mg L−1 citric acid) had only moderate effects on viability and regrowth of the microalgae since, after a short post-treatment delay of a few days, they reached final cell densities similar to that obtained for microalgae cultures that were not spiked. The decontamination was effective as no regrowth of rotifers was observed in the microalgae cultures after treatment. The efficacy of the citrate-modified photo-Fenton treatment was also studied with a higher starting concentration of 20 rotifers mL−1 and was compared with a solar light/H2O2 treatment. Results show that both treatments had similar efficacies on the rotifer elimination, but that the citrate-modified photo-Fenton treatment had a lower negative impact on the regrowth of microalgae than the solar light/H2O2 treatment. However, when microalgae cultures were spiked with 20 rotifers mL−1, rotifers were only partially inactivated and post-treatment regrowth occurred, which highlights the importance to apply the photo-Fenton process at an early stage of a contamination to achieve full rotifer elimination. In any case, a contamination with 5 rotifers mL−1 is already a significant threat as numbers above 1000 rotifers mL−1 were reached after 14 days and caused the microalgae culture to fail. Overall, our treatment suggests that the citrate-modified solar photo-Fenton process is an environmentally friendly solution to support the maintenance of contaminant-free microalgal cultures.
URI:	https://digitalcollection.zhaw.ch/handle/11475/25997
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Biogas2Algae
Appears in collections:	Publikationen Life Sciences und Facility Management

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Pulgarin, A., Decker, J., Chen, J., Giannakis, S., Ludwig, C., Refardt, D., & Pick, H. (2022). Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH. Water Research, 226(119301). https://doi.org/10.1016/j.watres.2022.119301

Pulgarin, A. et al. (2022) ‘Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH’, Water Research, 226(119301). Available at: https://doi.org/10.1016/j.watres.2022.119301.

A. Pulgarin et al., “Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH,” Water Research, vol. 226, no. 119301, 2022, doi: 10.1016/j.watres.2022.119301.

PULGARIN, Adrian, Jérémie DECKER, Jiahua CHEN, Stefanos GIANNAKIS, Christian LUDWIG, Dominik REFARDT und Horst PICK, 2022. Effective removal of the rotifer Brachionus calyciflorus from a Chlorella vulgaris microalgal culture by homogeneous solar photo-Fenton at neutral pH. Water Research. 2022. Bd. 226, Nr. 119301. DOI 10.1016/j.watres.2022.119301

Pulgarin, Adrian, Jérémie Decker, Jiahua Chen, Stefanos Giannakis, Christian Ludwig, Dominik Refardt, and Horst Pick. 2022. “Effective Removal of the Rotifer Brachionus Calyciflorus from a Chlorella Vulgaris Microalgal Culture by Homogeneous Solar Photo-Fenton at Neutral pH.” Water Research 226 (119301). https://doi.org/10.1016/j.watres.2022.119301.

Pulgarin, Adrian, et al. “Effective Removal of the Rotifer Brachionus Calyciflorus from a Chlorella Vulgaris Microalgal Culture by Homogeneous Solar Photo-Fenton at Neutral pH.” Water Research, vol. 226, no. 119301, 2022, https://doi.org/10.1016/j.watres.2022.119301.