Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility

Widmer, Susanne; Reber, Michael J.; Müller, Patrick; Housecroft, Catherine E.; Constable, Edwin C.; Rossi, René M.; Brühwiler, Dominik; Scherer, Lukas J.; Boesel, Luciano F.

doi:10.21256/zhaw-3561

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-3561

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility
Authors:	Widmer, Susanne Reber, Michael J. Müller, Patrick Housecroft, Catherine E. Constable, Edwin C. Rossi, René M. Brühwiler, Dominik Scherer, Lukas J. Boesel, Luciano F.
DOI:	10.21256/zhaw-3561 10.1039/C5AN00850F
Published in:	The Analyst
Volume(Issue):	140
Issue:	15
Page(s):	5324
Pages to:	5334
Issue Date:	2015
Publisher / Ed. Institution:	Royal Society of Chemistry
ISSN:	0003-2654 1364-5528
Language:	English
Subjects:	Sensor; Mesopores; SBA-15; Ammonia
Subject (DDC):	540: Chemistry
Abstract:	Fluorescein and rhodamine B modified mesoporous silica particles were synthesized by post-grafting and co-condensation approaches. The materials exhibited different pore size distributions, particle shapes and sizes. The materials were characterized by nitrogen sorption, scanning electron microscopy and fluorescence spectroscopy. The Förster resonance energy transfer between the selected dye pair was explored for the different materials by exposure to various concentrations of gaseous ammonia. A logarithmic increase in rhodamine B emission with increasing ammonia concentration was observed for both post-grafted and co-condensed materials. The dye accessibility by ammonia gas in the silica framework of mesoporous materials was evaluated by using a flow cell gas sensor setup built in-house. Response to ammonia gas and recovery with nitrogen gas are explained by comparing the structure properties and dye loading of the materials. The post-grafted dye modified silica showed better performance in terms of reversibility and recovery.
Further description:	Open Access Artikel
URI:	https://digitalcollection.zhaw.ch/handle/11475/2604
Fulltext version:	Published version
License (according to publishing contract):	CC BY 3.0: Attribution 3.0 Unported
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Published as part of the ZHAW project:	Molecular Alignment Chips (MACs)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Widmer, S., Reber, M. J., Müller, P., Housecroft, C. E., Constable, E. C., Rossi, R. M., Brühwiler, D., Scherer, L. J., & Boesel, L. F. (2015). Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility. The Analyst, 140(15), 5324–5334. https://doi.org/10.21256/zhaw-3561

Widmer, S. et al. (2015) ‘Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility’, The Analyst, 140(15), pp. 5324–5334. Available at: https://doi.org/10.21256/zhaw-3561.

S. Widmer et al., “Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility,” The Analyst, vol. 140, no. 15, pp. 5324–5334, 2015, doi: 10.21256/zhaw-3561.

WIDMER, Susanne, Michael J. REBER, Patrick MÜLLER, Catherine E. HOUSECROFT, Edwin C. CONSTABLE, René M. ROSSI, Dominik BRÜHWILER, Lukas J. SCHERER und Luciano F. BOESEL, 2015. Incorporation of a FRET dye pair into mesoporous materials : a comparison of fluorescence spectra, FRET activity and dye accessibility. The Analyst. 2015. Bd. 140, Nr. 15, S. 5324–5334. DOI 10.21256/zhaw-3561

Widmer, Susanne, Michael J. Reber, Patrick Müller, Catherine E. Housecroft, Edwin C. Constable, René M. Rossi, Dominik Brühwiler, Lukas J. Scherer, and Luciano F. Boesel. 2015. “Incorporation of a FRET Dye Pair into Mesoporous Materials : A Comparison of Fluorescence Spectra, FRET Activity and Dye Accessibility.” The Analyst 140 (15): 5324–34. https://doi.org/10.21256/zhaw-3561.

Widmer, Susanne, et al. “Incorporation of a FRET Dye Pair into Mesoporous Materials : A Comparison of Fluorescence Spectra, FRET Activity and Dye Accessibility.” The Analyst, vol. 140, no. 15, 2015, pp. 5324–34, https://doi.org/10.21256/zhaw-3561.