Publication type: | Article in scientific journal |
Type of review: | Not specified |
Title: | An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium |
Authors: | Rosenthal, Martin Immanuel Wolfram, Evelyn Meier, Beat |
Published in: | Pharmeuropa Bio & Scientific Notes |
Page(s): | 92 |
Pages to: | 102 |
Issue Date: | 2014 |
Publisher / Ed. Institution: | EDQM |
ISSN: | 2075-2164 2075-2504 |
Language: | English |
Subjects: | Senna; Pharmacopeia; Sennoside; Anthranoid |
Subject (DDC): | 615: Pharmacology and therapeutics |
Abstract: | Introduction: The current Ph. Eur. monographs for senna pods, senna leaf and senna leaf dry extract standardised describe a photometric assay based on the Bornträger reaction to determine hydroxyanthracene glycosides, calculated as sennoside B. The method is timeconsuming, unspecific for sennosides and the precision is not adequate for a modern assay. Aim: The photometric method shall therefore be replaced by a modern HPLC method. About 70 % of the total anthrachinone content in herbal drugs of senna species is due to sennoside A and sennoside B. These substances are therefore suitable for the standardisation of Senna products. The Japanese Pharmacopoeia (JP) already describes an HPLC method to determine sennoside A and sennoside B in the monograph for senna leaf. It uses ion-pair chromatography with tetraheptylammoniumbromide. The procedure described in the monograph has a runtime of 70 min. Method: The adapted and validated method described here uses solid-phase extraction (SPE) which allows a selective sample preparation by using an anion exchange phase. A conventional RP C18 column Tosh TSKgel ODS-80TS (4.6 mm × 150 mm), 5 μm, was used as stationary phase and acetonitrile for chromatography R, water R, phosphoric acid R (200:800:1 V/V/V) as mobile phase. The flow rate was 1.2 mL/min, the column temperature 40°C, the detection wavelength 380 nm, and the injection volume 20 μL. The runtime is 10 min, the chromatogram shows 2 peaks due to sennoside A/B and 2 additional smaller compounds. One of them is rhein-8-O-glucoside. Results: The procedure has been successfully validated according to ICH guidelines. We analysed 6 batches of Senna. The pods (Senna angustifolia) showed a total content of sennoside A and B of 1.74-2.76% m/m and the content of senna leaves was clearly lower with 1.07-1.19% m/m, respectively. Conclusion: The suggested method is considered to be suitable to determine sennoside A and sennoside B in senna leaves and senna pods. The consideration is based on the performed validation and on the results for the analysed samples. A short run time and better resolution are clear advantages of the suggested method, compared to other methods. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/3053 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Rosenthal, M. I., Wolfram, E., & Meier, B. (2014). An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium. Pharmeuropa Bio & Scientific Notes, 92–102.
Rosenthal, M.I., Wolfram, E. and Meier, B. (2014) ‘An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium’, Pharmeuropa Bio & Scientific Notes, pp. 92–102.
M. I. Rosenthal, E. Wolfram, and B. Meier, “An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium,” Pharmeuropa Bio & Scientific Notes, pp. 92–102, 2014.
ROSENTHAL, Martin Immanuel, Evelyn WOLFRAM und Beat MEIER, 2014. An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium. Pharmeuropa Bio & Scientific Notes. 2014. S. 92–102
Rosenthal, Martin Immanuel, Evelyn Wolfram, and Beat Meier. 2014. “An HPLC Method to Determine Sennoside A and Sennoside B in Sennae Fructus and Sennae Folium.” Pharmeuropa Bio & Scientific Notes, 92–102.
Rosenthal, Martin Immanuel, et al. “An HPLC Method to Determine Sennoside A and Sennoside B in Sennae Fructus and Sennae Folium.” Pharmeuropa Bio & Scientific Notes, 2014, pp. 92–102.
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