Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation
Authors: Schwendimann, Livia Maya
Kauf, Peter
Fieseler, Lars
Gantenbein-Demarchi, Corinne
Miescher Schwenninger, Susanne
DOI: 10.1016/j.mimet.2015.05.022
Published in: Journal of Microbiological Methods
Volume(Issue): 115
Pages: 94
Pages to: 99
Issue Date: Aug-2015
Publisher / Ed. Institution: Elsevier
ISSN: 0167-7012
Language: English
Subjects: Lactobacillus fermentum; Lactobacillus plantarum; QPCR; Coca bean fermentation
Subject (DDC): 664: Food technology
Abstract: To monitor dominant species of lactic acid bacteria during cocoa bean fermentation, i.e. Lactobacillus plantarum and Lactobacillus fermentum, a fast and reliable culture-independent qPCR assay was developed. A modified DNA isolation procedure using a commercial kit followed by two species-specific qPCR assays resulted in 100% sensitivity for L. plantarum and L. fermentum. Kruskal–Wallis and post-hoc analyses of data obtained from experiments with cocoa beans that were artificially spiked with decimal concentrations of L. plantarum and L. fermentum strains allowed the calculation of a regression line suitable for the estimation of both species with a detection limit of 3 to 4 Log cells/g cocoa beans. This process was successfully tested for efficacy through the analyses of samples from laboratory-scale cocoa bean fermentations with both the qPCR assay and a culture-dependent method which resulted in comparable results.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Appears in collections:Publikationen Life Sciences und Facility Management

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