Publication type: Conference poster
Type of review: Not specified
Title: Biofilms in metalworking fluids
Authors: Berner, Florian
Inauen, Silvio
Rüfenacht, Andrea
Krebs, Walter
Conference details: Biofilms IV - International Conference: «Communities Bridging Disciplines», Winchester, United Kingdom, 2010
Issue Date: 2010
Language: English
Subjects: Kühlschmierstoffe; Biofilme; Mikrobiologie
Subject (DDC): 570: Biology
Abstract: Metalworking fluids play a critical role whenever metal is shaped in modern tool machines. The cooling and lubrication provided by these oil-water emulsions are important factors in improving the quality of the product and the lifetime of the tools. As these machines are open systems, the metalworking fluids are colonized and eventually degraded by microorganisms. Consequently industrial research has a considerable interest in the microbiology of these systems. However, most previous studies have focused on the planctonic organisms of the liquid phase, with little work done on biofilms. In this CTI-funded project an existing industrial test reactor for metalworking fluids (designed by Blaser Swisslube AG) was adapted for biofilm cultivation. Biofilms were cultivated in different Blasocut®Bioconcept formulations, a metalworking fluid designed to maintain stability by allowing a dominant population of P. pseudoalcaligenes that prevents growth of other species. The reactors were monitored for up to eight weeks for planctonic and biofilm growth, which was quantified using classical cultivation methods and total mass. Growth was reproducible independent of the test coupons material (stainless steel or aluminium). Different biofilm quantities were shown for different Blasocut®Bioconcept formulations, strongly corresponding with planctonic bacterial densities. In order to study the microbial diversity of the biofilm and determine how it differed from the planctonic phase, samples were also analysed by mass spectroscopy (MALDI-TOF), quantitative PCR and denaturing gradient gel electrophoresis.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Published as part of the ZHAW project: Analyse und Kontrolle von Biofilmen in Kühlschmierstoffsystemen
Appears in collections:Publikationen Life Sciences und Facility Management

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