1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4807
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLooser, Verena-
dc.contributor.authorBrühlmann, Béla-
dc.contributor.authorBumbak, Fabian-
dc.contributor.authorStenger, Chantal-
dc.contributor.authorCosta, Merixtell-
dc.contributor.authorCamattari, Andrea-
dc.contributor.authorFotiadis, Dimitrios-
dc.contributor.authorKovar, Karin-
dc.date.accessioned2018-09-20T12:30:25Z-
dc.date.available2018-09-20T12:30:25Z-
dc.date.issued2015-05-25-
dc.identifier.issn0734-9750de_CH
dc.identifier.issn1873-1899de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/10880-
dc.description.abstractPichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory proteins such as chaperones, engineering of glycosylation and secretory pathways, etc. However, the physiological effects of different cultivation strategies are often difficult to separate from the molecular effects of the gene construct (e.g., cellular stress through over-expression or incorrect post-translational processing). Hence, overall system optimisation is difficult, even though it is urgently required in order to describe and understand the behaviour of new molecular constructs. This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors. The relationship between specific formation rates of secreted recombinant proteins, qp, and specific growth rates, μ, has been analysed in a conceptual attempt to compare different systems, particularly those based on AOX1/methanol and GAP/glucose, and this has now evolved into a pivotal concept for bioprocess engineering of P. pastoris.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofBiotechnology Advancesde_CH
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/de_CH
dc.subjectPichia pastorisde_CH
dc.subjectRecombinant proteinde_CH
dc.subjectProduction process developmentde_CH
dc.subjectFermentationde_CH
dc.subjectProductivityde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleCultivation strategies to enhance productivity of Pichia pastoris : a reviewde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.21256/zhaw-4807-
dc.identifier.doi10.1016/j.biotechadv.2015.05.008de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end1193de_CH
zhaw.pages.start1177de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume33de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedBiokatalysede_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2015_Looser_Cultivation_Strategies_To_Enhance.pdf1.36 MBAdobe PDFThumbnail
View/Open
Show simple item record
Looser, V., Brühlmann, B., Bumbak, F., Stenger, C., Costa, M., Camattari, A., Fotiadis, D., & Kovar, K. (2015). Cultivation strategies to enhance productivity of Pichia pastoris : a review. Biotechnology Advances, 33, 1177–1193. https://doi.org/10.21256/zhaw-4807
Looser, V. et al. (2015) ‘Cultivation strategies to enhance productivity of Pichia pastoris : a review’, Biotechnology Advances, 33, pp. 1177–1193. Available at: https://doi.org/10.21256/zhaw-4807.
V. Looser et al., “Cultivation strategies to enhance productivity of Pichia pastoris : a review,” Biotechnology Advances, vol. 33, pp. 1177–1193, May 2015, doi: 10.21256/zhaw-4807.
LOOSER, Verena, Béla BRÜHLMANN, Fabian BUMBAK, Chantal STENGER, Merixtell COSTA, Andrea CAMATTARI, Dimitrios FOTIADIS und Karin KOVAR, 2015. Cultivation strategies to enhance productivity of Pichia pastoris : a review. Biotechnology Advances. 25 Mai 2015. Bd. 33, S. 1177–1193. DOI 10.21256/zhaw-4807
Looser, Verena, Béla Brühlmann, Fabian Bumbak, Chantal Stenger, Merixtell Costa, Andrea Camattari, Dimitrios Fotiadis, and Karin Kovar. 2015. “Cultivation Strategies to Enhance Productivity of Pichia Pastoris : A Review.” Biotechnology Advances 33 (May): 1177–93. https://doi.org/10.21256/zhaw-4807.
Looser, Verena, et al. “Cultivation Strategies to Enhance Productivity of Pichia Pastoris : A Review.” Biotechnology Advances, vol. 33, May 2015, pp. 1177–93, https://doi.org/10.21256/zhaw-4807.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.