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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4838
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dc.contributor.authorKriszt, Rókus-
dc.contributor.authorArai, Satoshi-
dc.contributor.authorItoh, Hideki-
dc.contributor.authorLee, Michelle H.-
dc.contributor.authorGoralczyk, Anna G.-
dc.contributor.authorAng, Xiu Min-
dc.contributor.authorCypess, Aaron M.-
dc.contributor.authorWhite, Andrew P.-
dc.contributor.authorShamsi, Farnaz-
dc.contributor.authorXue, Ruidan-
dc.contributor.authorLee, Jung Yeol-
dc.contributor.authorLee, Sung-Chan-
dc.contributor.authorHou, Yanyan-
dc.contributor.authorKitaguchi, Tetsuya-
dc.contributor.authorSudhaharan, Thankiah-
dc.contributor.authorIshiwata, Shin’ichi-
dc.contributor.authorLane, E. Birgitte-
dc.contributor.authorChang, Young-Tae-
dc.contributor.authorTseng, Yu-Hua-
dc.contributor.authorSuzuki, Madoka-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2018-10-24T09:27:20Z-
dc.date.available2018-10-24T09:27:20Z-
dc.date.issued2017-
dc.identifier.issn2045-2322de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12094-
dc.description.abstractThe identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.rightshttps://creativecommons.org/licenses/by/4.0/de_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleOptical visualisation of thermogenesis in stimulated singlecell brown adipocytesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.21256/zhaw-4838-
dc.identifier.doi10.1038/s41598-017-00291-9de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Kriszt, R., Arai, S., Itoh, H., Lee, M. H., Goralczyk, A. G., Ang, X. M., Cypess, A. M., White, A. P., Shamsi, F., Xue, R., Lee, J. Y., Lee, S.-C., Hou, Y., Kitaguchi, T., Sudhaharan, T., Ishiwata, S., Lane, E. B., Chang, Y.-T., Tseng, Y.-H., et al. (2017). Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes. Scientific Reports. https://doi.org/10.21256/zhaw-4838
Kriszt, R. et al. (2017) ‘Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes’, Scientific Reports [Preprint]. Available at: https://doi.org/10.21256/zhaw-4838.
R. Kriszt et al., “Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes,” Scientific Reports, 2017, doi: 10.21256/zhaw-4838.
KRISZT, Rókus, Satoshi ARAI, Hideki ITOH, Michelle H. LEE, Anna G. GORALCZYK, Xiu Min ANG, Aaron M. CYPESS, Andrew P. WHITE, Farnaz SHAMSI, Ruidan XUE, Jung Yeol LEE, Sung-Chan LEE, Yanyan HOU, Tetsuya KITAGUCHI, Thankiah SUDHAHARAN, Shin’ichi ISHIWATA, E. Birgitte LANE, Young-Tae CHANG, Yu-Hua TSENG, Madoka SUZUKI und Michael RAGHUNATH, 2017. Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes. Scientific Reports. 2017. DOI 10.21256/zhaw-4838
Kriszt, Rókus, Satoshi Arai, Hideki Itoh, Michelle H. Lee, Anna G. Goralczyk, Xiu Min Ang, Aaron M. Cypess, et al. 2017. “Optical Visualisation of Thermogenesis in Stimulated Singlecell Brown Adipocytes.” Scientific Reports. https://doi.org/10.21256/zhaw-4838.
Kriszt, Rókus, et al. “Optical Visualisation of Thermogenesis in Stimulated Singlecell Brown Adipocytes.” Scientific Reports, 2017, https://doi.org/10.21256/zhaw-4838.


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