1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBadowski, Cedric-
dc.contributor.authorIskander, Aneesa-
dc.contributor.authorGaspar, Diana-
dc.contributor.authorZeugolis, Dimitrios I.-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2018-10-24T09:58:03Z-
dc.date.available2018-10-24T09:58:03Z-
dc.date.issued2018-
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12106-
dc.description.abstractMacromolecular crowding (MMC) is an intrinsic and ubiquitous feature in biological cells. We find MMC in the first bacterial cell and see it culminating in the intricate extracellular matrix (ECM) that evolved in multicellular organisms. Research work on MMC started with the observation that biological cellular systems are crammed with macromolecules. The interior of cells is teeming with enzymes, transport systems, and nucleotide assemblies. In addition, eukaryotic cells possess a three-layered cytoskeleton adding confinement to an already packed cytoplasm. Likewise, the extracellular space of multicellular organisms comprises an ECM consisting of fibrillar proteins, such as collagen or elastin, surrounded by an amorphous gel-like ground substance glycoproteins and proteoglycans and their hydration shells. Together, they provide mechanical resilience to the tissues of vertebrates while forming a crowded and structural microenvironment that in turn creates confinement for other macromolecules. Surprisingly, most biochemical and cell culture experiments are still done in non-crowded, highly aqueous solutions. Here, we shall discuss the shortcomings of contemporary cell culture and emphasize the benefits of applying MMC to cell culture models of tissues. MMC can be achieved by adding water-soluble macromolecules to the culture medium. Not only is this technically feasible, it also moves in vitro biology toward a higher physiological level, allowing the design of more meaningful cell-based assays and enabling tissue engineering of matured and physiologically relevant tissue-like assemblies.de_CH
dc.language.isoende_CH
dc.publisherSpringerde_CH
dc.relation.ispartofTissue engineering and regeneration : cell engineering and regenerationde_CH
dc.relation.ispartofseriesReference Series in Biomedical Engineeringde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.titleMolecular Crowding – (in Cell Culture)de_CH
dc.typeBuchbeitragde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1007/978-3-319-37076-7_50-1de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.publication.reviewEditorial reviewde_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Badowski, C., Iskander, A., Gaspar, D., Zeugolis, D. I., & Raghunath, M. (2018). Molecular Crowding – (in Cell Culture). In Tissue engineering and regeneration : cell engineering and regeneration. Springer. https://doi.org/10.1007/978-3-319-37076-7_50-1
Badowski, C. et al. (2018) ‘Molecular Crowding – (in Cell Culture)’, in Tissue engineering and regeneration : cell engineering and regeneration. Springer. Available at: https://doi.org/10.1007/978-3-319-37076-7_50-1.
C. Badowski, A. Iskander, D. Gaspar, D. I. Zeugolis, and M. Raghunath, “Molecular Crowding – (in Cell Culture),” in Tissue engineering and regeneration : cell engineering and regeneration, Springer, 2018. doi: 10.1007/978-3-319-37076-7_50-1.
BADOWSKI, Cedric, Aneesa ISKANDER, Diana GASPAR, Dimitrios I. ZEUGOLIS und Michael RAGHUNATH, 2018. Molecular Crowding – (in Cell Culture). In: Tissue engineering and regeneration : cell engineering and regeneration. Springer
Badowski, Cedric, Aneesa Iskander, Diana Gaspar, Dimitrios I. Zeugolis, and Michael Raghunath. 2018. “Molecular Crowding – (in Cell Culture).” In Tissue Engineering and Regeneration : Cell Engineering and Regeneration. Springer. https://doi.org/10.1007/978-3-319-37076-7_50-1.
Badowski, Cedric, et al. “Molecular Crowding – (in Cell Culture).” Tissue Engineering and Regeneration : Cell Engineering and Regeneration, Springer, 2018, https://doi.org/10.1007/978-3-319-37076-7_50-1.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.