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dc.contributor.authorRashid, Rafi-
dc.contributor.authorChee, Stella Min Ling-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorWohland, Thorsten-
dc.date.accessioned2018-10-26T13:55:16Z-
dc.date.available2018-10-26T13:55:16Z-
dc.date.issued2015-
dc.identifier.issn1478-3967de_CH
dc.identifier.issn1478-3975de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12189-
dc.description.abstractMacromolecular crowding (MMC) has been used in various in vitro experimental systems to mimic in vivo physiology. This is because the crowded cytoplasm of cells contains many different types of solutes dissolved in an aqueous medium. MMC in the extracellular microenvironment is involved in maintaining stem cells in their undifferentiated state (niche) as well as in aiding their differentiation after they have travelled to new locations outside the niche. MMC at physiologically relevant fractional volume occupancies (FVOs) significantly enhances the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells during chemically induced adipogenesis. The mechanism by which MMC produces this enhancement is not entirely known. In the context of extracellular collagen deposition, we have recently reported the importance of optimizing the FVO while minimizing the bulk viscosity. Two opposing properties will determine the net rate of a biochemical reaction: the negative effect of bulk viscosity and the positive effect of the excluded volume, the latter being expressed by the FVO. In this study we have looked more closely at the effect of viscosity on reaction rates. We have used fluorimetry to measure the rate of actin polymerization and fluorescence correlation spectroscopy (FCS) to measure diffusion of various probes in solutions containing the crowder Ficoll at physiological concentrations. Similar to its effect on collagen, Ficoll enhanced the actin polymerization rate despite increasing the bulk viscosity. Our FCS measurements reveal a relatively minor component of anomalous diffusion. In addition, our measurements do suggest that microviscosity becomes relevant in a crowded environment. We ruled out bulk viscosity as a cause of the rate enhancement by performing the actin polymerization assay in glycerol. These opposite effects of Ficoll and glycerol led us to conclude that microviscosity becomes relevant at the length scale of the reacting molecules within a crowded microenvironment. The excluded volume effect (arising from crowding) increases the effective concentration of actin, which increases the reaction rate, while the microviscosity does not increase sufficiently to lower the reaction rate. This study reveals finer details about the mechanism of MMC.de_CH
dc.language.isoende_CH
dc.publisherIOP Publishingde_CH
dc.relation.ispartofPhysical Biologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleMacromolecular crowding gives rise to microviscosity, anomalous diffusion and accelerated actin polymerizationde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1088/1478-3975/12/3/034001de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.start034001de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Rashid, R., Chee, S. M. L., Raghunath, M., & Wohland, T. (2015). Macromolecular crowding gives rise to microviscosity, anomalous diffusion and accelerated actin polymerization. Physical Biology, 12(3), 34001. https://doi.org/10.1088/1478-3975/12/3/034001
Rashid, R. et al. (2015) ‘Macromolecular crowding gives rise to microviscosity, anomalous diffusion and accelerated actin polymerization’, Physical Biology, 12(3), p. 034001. Available at: https://doi.org/10.1088/1478-3975/12/3/034001.
R. Rashid, S. M. L. Chee, M. Raghunath, and T. Wohland, “Macromolecular crowding gives rise to microviscosity, anomalous diffusion and accelerated actin polymerization,” Physical Biology, vol. 12, no. 3, p. 034001, 2015, doi: 10.1088/1478-3975/12/3/034001.
RASHID, Rafi, Stella Min Ling CHEE, Michael RAGHUNATH und Thorsten WOHLAND, 2015. Macromolecular crowding gives rise to microviscosity, anomalous diffusion and accelerated actin polymerization. Physical Biology. 2015. Bd. 12, Nr. 3, S. 034001. DOI 10.1088/1478-3975/12/3/034001
Rashid, Rafi, Stella Min Ling Chee, Michael Raghunath, and Thorsten Wohland. 2015. “Macromolecular Crowding Gives Rise to Microviscosity, Anomalous Diffusion and Accelerated Actin Polymerization.” Physical Biology 12 (3): 34001. https://doi.org/10.1088/1478-3975/12/3/034001.
Rashid, Rafi, et al. “Macromolecular Crowding Gives Rise to Microviscosity, Anomalous Diffusion and Accelerated Actin Polymerization.” Physical Biology, vol. 12, no. 3, 2015, p. 34001, https://doi.org/10.1088/1478-3975/12/3/034001.


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