Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-4752
Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs |
Authors: | H. Lee, Michelle Goralczyk, Anna G. Kriszt, Rókus Ang, Xiu Min Badowski, Cedric Li, Ying Summers, Scott A. Toh, Sue-Anne Yassin, M. Shabeer Shabbir, Asim Sheppard, Allan Raghunath, Michael |
DOI: | 10.21256/zhaw-4752 10.1038/srep21173 |
Published in: | Scientific Reports |
Volume(Issue): | 6 |
Issue: | 21173 |
Issue Date: | 2016 |
Publisher / Ed. Institution: | Nature Publishing Group |
ISSN: | 2045-2322 |
Language: | English |
Subject (DDC): | 571: Physiology and related subjects 610: Medicine and health |
Abstract: | Key to realizing the diagnostic and therapeutic potential of human brown/brite adipocytes is the identification of a renewable, easily accessible and safe tissue source of progenitor cells, and an efficacious in vitro differentiation protocol. We show that macromolecular crowding (MMC) facilitates brown adipocyte differentiation in adult human bone marrow mesenchymal stem cells (bmMSCs), as evidenced by substantially upregulating uncoupling protein 1 (UCP1) and uncoupled respiration. Moreover, MMC also induced ‘browning’ in bmMSC-derived white adipocytes. Mechanistically, MMC creates a 3D extracellular matrix architecture enshrouding maturing adipocytes in a collagen IV cocoon that is engaged by paxillin-positive focal adhesions also at the apical side of cells, without contact to the stiff support structure. This leads to an enhanced matrix-cell signaling, reflected by increased phosphorylation of ATF2, a key transcription factor in UCP1 regulation. Thus, tuning the dimensionality of the microenvironment in vitro can unlock a strong brown potential dormant in bone marrow. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/12195 |
Fulltext version: | Published version |
License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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2016_Lee_et_al_ECM_microenvironment_unlocks_brown_adipogenic_potential.pdf | 3.71 MB | Adobe PDF | View/Open |
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H. Lee, M., Goralczyk, A. G., Kriszt, R., Ang, X. M., Badowski, C., Li, Y., Summers, S. A., Toh, S.-A., Yassin, M. S., Shabbir, A., Sheppard, A., & Raghunath, M. (2016). ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Scientific Reports, 6(21173). https://doi.org/10.21256/zhaw-4752
H. Lee, M. et al. (2016) ‘ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs’, Scientific Reports, 6(21173). Available at: https://doi.org/10.21256/zhaw-4752.
M. H. Lee et al., “ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs,” Scientific Reports, vol. 6, no. 21173, 2016, doi: 10.21256/zhaw-4752.
H. LEE, Michelle, Anna G. GORALCZYK, Rókus KRISZT, Xiu Min ANG, Cedric BADOWSKI, Ying LI, Scott A. SUMMERS, Sue-Anne TOH, M. Shabeer YASSIN, Asim SHABBIR, Allan SHEPPARD und Michael RAGHUNATH, 2016. ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Scientific Reports. 2016. Bd. 6, Nr. 21173. DOI 10.21256/zhaw-4752
H. Lee, Michelle, Anna G. Goralczyk, Rókus Kriszt, Xiu Min Ang, Cedric Badowski, Ying Li, Scott A. Summers, et al. 2016. “ECM Microenvironment Unlocks Brown Adipogenic Potential of Adult Human Bone Marrow-Derived MSCs.” Scientific Reports 6 (21173). https://doi.org/10.21256/zhaw-4752.
H. Lee, Michelle, et al. “ECM Microenvironment Unlocks Brown Adipogenic Potential of Adult Human Bone Marrow-Derived MSCs.” Scientific Reports, vol. 6, no. 21173, 2016, https://doi.org/10.21256/zhaw-4752.
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