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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4871
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dc.contributor.authorKlawitter, Marina-
dc.contributor.authorQuero, Lilian-
dc.contributor.authorKlasen, Juergen-
dc.contributor.authorGloess, Alexia N.-
dc.contributor.authorKlopprogge, Babette-
dc.contributor.authorHausmann, Oliver-
dc.contributor.authorBoos, Norbert-
dc.contributor.authorWuertz, Karin-
dc.date.accessioned2018-11-16T10:08:48Z-
dc.date.available2018-11-16T10:08:48Z-
dc.date.issued2012-
dc.identifier.issn1476-9255de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12905-
dc.description.abstractBackground: As proinflammatory cytokines seem to play a role in discogenic back pain, substances exhibiting anti-inflammatory effects on intervertebral disc cells may be used as minimal-invasive therapeutics for intradiscal/epidural injection. The purpose of this study was to investigate the anti-inflammatory and anti-catabolic potential of curcuma, which has been used in the Indian Ayurvedic medicine to treat multiple ailments for a long time. Methods: Human disc cells were treated with IL-1β to induce an inflammatory/catabolic cascade. Different extracts of curcuma as well as curcumin (= a component selected based on results with curcuma extracts and HPLC/MS analysis) were tested for their ability to reduce mRNA expression of proinflammatory cytokines and matrix degrading enzymes after 6 hours (real-time RT-PCR), followed by analysis of typical inflammatory signaling mechanisms such as NF-κB (Western Blot, Transcription Factor Assay), MAP kinases (Western Blot) and Toll-like receptors (real-time RT-PCR). Quantitative data was statistically analyzed using a Mann Whitney U test with a significance level of p < 0.05 (two-tailed). Results: Results indicate that the curcuma DMSO extract significantly reduced levels of IL-6, MMP1, MMP3 and MMP13. The DMSO-soluble component curcumin, whose occurrence within the DMSO extract was verified by HPLC/MS, reduced levels of IL-1β, IL-6, IL-8, MMP1, MMP3 and MMP13 and both caused an up-regulation of TNF-α. Pathway analysis indicated that curcumin did not show involvement of NF-κB, but down-regulated TLR2 expression and inhibited the MAP kinase JNK while activating p38 and ERK. Conclusions: Based on its anti-inflammatory and anti-catabolic effects, intradiscal injection of curcumin may be an attractive treatment alternative. However, whether the anti-inflammatory properties in vitro lead to analgesia in vivo will need to be confirmed in an appropriate animal model.de_CH
dc.language.isoende_CH
dc.publisherBioMed Centralde_CH
dc.relation.ispartofJournal of Inflammationde_CH
dc.rightshttp://creativecommons.org/licenses/by/2.0/de_CH
dc.subject.ddc580: Pflanzen (Botanik)de_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleCurcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activityde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.21256/zhaw-4871-
dc.identifier.doi10.1186/1476-9255-9-29de_CH
dc.identifier.pmid22909087de_CH
zhaw.funding.euNode_CH
zhaw.issue29de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume9de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Klawitter, M., Quero, L., Klasen, J., Gloess, A. N., Klopprogge, B., Hausmann, O., Boos, N., & Wuertz, K. (2012). Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity. Journal of Inflammation, 9(29). https://doi.org/10.21256/zhaw-4871
Klawitter, M. et al. (2012) ‘Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity’, Journal of Inflammation, 9(29). Available at: https://doi.org/10.21256/zhaw-4871.
M. Klawitter et al., “Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity,” Journal of Inflammation, vol. 9, no. 29, 2012, doi: 10.21256/zhaw-4871.
KLAWITTER, Marina, Lilian QUERO, Juergen KLASEN, Alexia N. GLOESS, Babette KLOPPROGGE, Oliver HAUSMANN, Norbert BOOS und Karin WUERTZ, 2012. Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity. Journal of Inflammation. 2012. Bd. 9, Nr. 29. DOI 10.21256/zhaw-4871
Klawitter, Marina, Lilian Quero, Juergen Klasen, Alexia N. Gloess, Babette Klopprogge, Oliver Hausmann, Norbert Boos, and Karin Wuertz. 2012. “Curcuma DMSO Extracts and Curcumin Exhibit an Anti-Inflammatory and Anti-Catabolic Effect on Human Intervertebral Disc Cells, Possibly by Influencing TLR2 Expression and JNK Activity.” Journal of Inflammation 9 (29). https://doi.org/10.21256/zhaw-4871.
Klawitter, Marina, et al. “Curcuma DMSO Extracts and Curcumin Exhibit an Anti-Inflammatory and Anti-Catabolic Effect on Human Intervertebral Disc Cells, Possibly by Influencing TLR2 Expression and JNK Activity.” Journal of Inflammation, vol. 9, no. 29, 2012, https://doi.org/10.21256/zhaw-4871.


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