Tripartite species interaction : eukarotic hosts suffer more from phage susceptible than from phage resistant bacteria

Wendling, Carolin Charlotte; Piecyk, Agnes; Refardt, Dominik; Chibani, Cynthia Maria; Hertel, Robert; Liesegang, Heiko; Bunk, Boyke; Overmann, Jörg; Roth, Olivia

doi:10.21256/zhaw-3456

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-3456

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Tripartite species interaction : eukarotic hosts suffer more from phage susceptible than from phage resistant bacteria
Authors:	Wendling, Carolin Charlotte Piecyk, Agnes Refardt, Dominik Chibani, Cynthia Maria Hertel, Robert Liesegang, Heiko Bunk, Boyke Overmann, Jörg Roth, Olivia
DOI:	10.21256/zhaw-3456 10.1186/s12862-017-0930-2
Published in:	BMC Evolutionary Biology
Volume(Issue):	17
Issue:	1
Page(s):	98
Issue Date:	2017
Publisher / Ed. Institution:	BioMed Central
ISSN:	1471-2148
Language:	English
Subjects:	Bacteriophages; Vibrio; Pipefish; Lysogeny
Subject (DDC):	570: Biology
Abstract:	Background: Evolutionary shifts in bacterial virulence are often associated with a third biological player, for instance temperate phages, that can act as hyperparasites. By integrating as prophages into the bacterial genome they can contribute accessory genes, which can enhance the fitness of their prokaryotic carrier (lysogenic conversion). Hyperparasitic influence in tripartite biotic interactions has so far been largely neglected in empirical host-parasite studies due to their inherent complexity. Here we experimentally address whether bacterial resistance to phages and bacterial harm to eukaryotic hosts is linked using a natural tri-partite system with bacteria of the genus Vibrio, temperate vibriophages and the pipefish Syngnathus typhle. We induced prophages from all bacterial isolates and constructed a three-fold replicated, fully reciprocal 75 × 75 phage-bacteria infection matrix. Results: According to their resistance to phages, bacteria could be grouped into three distinct categories: highly susceptible (HS-bacteria), intermediate susceptible (IS-bacteria), and resistant (R-bacteria). We experimentally challenged pipefish with three selected bacterial isolates from each of the three categories and determined the amount of viable Vibrio counts from infected pipefish and the expression of pipefish immune genes. While the amount of viable Vibrio counts did not differ between bacterial groups, we observed a significant difference in relative gene expression between pipefish infected with phage susceptible and phage resistant bacteria. Conclusion: These findings suggest that bacteria with a phage-susceptible phenotype are more harmful against a eukaryotic host, and support the importance of hyperparasitism and the need for an integrative view across more than two levels when studying host-parasite evolution.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1471
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Wendling, C. C., Piecyk, A., Refardt, D., Chibani, C. M., Hertel, R., Liesegang, H., Bunk, B., Overmann, J., & Roth, O. (2017). Tripartite species interaction : eukarotic hosts suffer more from phage susceptible than from phage resistant bacteria. BMC Evolutionary Biology, 17(1), 98. https://doi.org/10.21256/zhaw-3456

Wendling, C.C. et al. (2017) ‘Tripartite species interaction : eukarotic hosts suffer more from phage susceptible than from phage resistant bacteria’, BMC Evolutionary Biology, 17(1), p. 98. Available at: https://doi.org/10.21256/zhaw-3456.

C. C. Wendling et al., “Tripartite species interaction : eukarotic hosts suffer more from phage susceptible than from phage resistant bacteria,” BMC Evolutionary Biology, vol. 17, no. 1, p. 98, 2017, doi: 10.21256/zhaw-3456.

WENDLING, Carolin Charlotte, Agnes PIECYK, Dominik REFARDT, Cynthia Maria CHIBANI, Robert HERTEL, Heiko LIESEGANG, Boyke BUNK, Jörg OVERMANN und Olivia ROTH, 2017. Tripartite species interaction : eukarotic hosts suffer more from phage susceptible than from phage resistant bacteria. BMC Evolutionary Biology. 2017. Bd. 17, Nr. 1, S. 98. DOI 10.21256/zhaw-3456

Wendling, Carolin Charlotte, Agnes Piecyk, Dominik Refardt, Cynthia Maria Chibani, Robert Hertel, Heiko Liesegang, Boyke Bunk, Jörg Overmann, and Olivia Roth. 2017. “Tripartite Species Interaction : Eukarotic Hosts Suffer More from Phage Susceptible than from Phage Resistant Bacteria.” BMC Evolutionary Biology 17 (1): 98. https://doi.org/10.21256/zhaw-3456.

Wendling, Carolin Charlotte, et al. “Tripartite Species Interaction : Eukarotic Hosts Suffer More from Phage Susceptible than from Phage Resistant Bacteria.” BMC Evolutionary Biology, vol. 17, no. 1, 2017, p. 98, https://doi.org/10.21256/zhaw-3456.