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dc.contributor.authorThurnheer, Anina-
dc.contributor.authorJosuran, Roland-
dc.contributor.authorMüller, Christian-
dc.contributor.authorAvondet, Marc-André-
dc.contributor.authorZaborosch, Christiane-
dc.date.accessioned2019-02-11T15:35:11Z-
dc.date.available2019-02-11T15:35:11Z-
dc.date.issued2015-
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/15269-
dc.descriptionOral Presentationde_CH
dc.description.abstractRicin is a potent toxin from the plant Ricinus communis which is included on list 1 of the Chemical Weapons Convention because of its high toxicity and easy availability. Ricin is present as a 59 kDa glycoprotein in the seeds of Ricinus communis and consists of two disulphide-linked subunits. N-glycan species of ricin were identified by enzymatic cleavage from the protein part, separation of the glycan species by porous graphitized carbon chromatography (PGCC) and detection with an electrospray ionization quadrupole time‐of-flight mass spectrometer (ESI‐Q‐TOF‐MS). Glycosylation sites in the protein were identified by capillary electrophoresis separation of proteolytically digested glycopeptides coupled to a mass spectrometer (CE‐MS). In addition, a CE method was established for the analysis of the monosaccharide composition of glycans with detection by means of LED‐induced fluorescence (CE-LEDIF). Ricin was shown to contain high‐mannose type glycans with additional monosaccharides, such as xylose, and different linkages of monosaccharide building locks compared to animal glycoproteins. Ten different high-mannose glycan species of ricin were identified using PGCC‐MS analysis. Linkages of the monosaccharide building blocks of the glycans were further characterized by exoglycosidase digestion with α(1‐2)‐mannosidase and α(1‐6)‐mannosidase.de_CH
dc.language.isoende_CH
dc.rightsNot specifiedde_CH
dc.subjectMass spectrometryde_CH
dc.subjectGlycan analysisde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleMass spectrometry-based glycan analysis of the glycoprotein ricinde_CH
dc.typeKonferenz: Sonstigesde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.conference.detailsChanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.publication.reviewNot specifiedde_CH
zhaw.funding.zhawAufbau der Kompetenz und Machbarkeitsstudie zur Analytik pflanzlicher Glykanstrukturen und in Pflanzenzellen hergestellter Glykoproteinede_CH
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Thurnheer, A., Josuran, R., Müller, C., Avondet, M.-A., & Zaborosch, C. (2015). Mass spectrometry-based glycan analysis of the glycoprotein ricin. Chanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015.
Thurnheer, A. et al. (2015) ‘Mass spectrometry-based glycan analysis of the glycoprotein ricin’, in Chanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015.
A. Thurnheer, R. Josuran, C. Müller, M.-A. Avondet, and C. Zaborosch, “Mass spectrometry-based glycan analysis of the glycoprotein ricin,” in Chanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015, 2015.
THURNHEER, Anina, Roland JOSURAN, Christian MÜLLER, Marc-André AVONDET und Christiane ZABOROSCH, 2015. Mass spectrometry-based glycan analysis of the glycoprotein ricin. In: Chanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015. Conference presentation. 2015
Thurnheer, Anina, Roland Josuran, Christian Müller, Marc-André Avondet, and Christiane Zaborosch. 2015. “Mass Spectrometry-Based Glycan Analysis of the Glycoprotein Ricin.” Conference presentation. In Chanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015.
Thurnheer, Anina, et al. “Mass Spectrometry-Based Glycan Analysis of the Glycoprotein Ricin.” Chanalysis - Meeting of the Division Analytical Sciences of the Swiss Chemical Society (SCG/DAS), Beatenberg, 10 April 2015, 2015.


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