Automation of 3D cell culture using chemically defined hydrogels

Rimann, Markus; Graf-Hausner, Ursula; Patocchi-Tenzer, Isabel; Angres, Brigitte; Braum, Susanne

doi:10.1177/2211068213508651

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Automation of 3D cell culture using chemically defined hydrogels
Authors:	Rimann, Markus Graf-Hausner, Ursula Patocchi-Tenzer, Isabel Angres, Brigitte Braum, Susanne
DOI:	10.1177/2211068213508651
Published in:	SLAS Technology: Translating Life Sciences Innovation
Volume(Issue):	19
Issue:	2
Page(s):	191
Pages to:	197
Issue Date:	2014
Publisher / Ed. Institution:	Sage
ISSN:	2472-6303 2472-6311
Language:	English
Subject (DDC):	660.6: Biotechnology
Abstract:	Drug development relies on high-throughput screening involving cell-based assays. Most of the assays are still based on cells grown in monolayer rather than in three-dimensional (3D) formats, although cells behave more in vivo–like in 3D. To exemplify the adoption of 3D techniques in drug development, this project investigated the automation of a hydrogel-based 3D cell culture system using a liquid-handling robot. The hydrogel technology used offers high flexibility of gel design due to a modular composition of a polymer network and bioactive components. The cell inert degradation of the gel at the end of the culture period guaranteed the harmless isolation of live cells for further downstream processing. Human colon carcinoma cells HCT-116 were encapsulated and grown in these dextran-based hydrogels, thereby forming 3D multicellular spheroids. Viability and DNA content of the cells were shown to be similar in automated and manually produced hydrogels. Furthermore, cell treatment with toxic Taxol concentrations (100 nM) had the same effect on HCT-116 cell viability in manually and automated hydrogel preparations. Finally, a fully automated dose-response curve with the reference compound Taxol showed the potential of this hydrogel-based 3D cell culture system in advanced drug development.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1608
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Rimann, M., Graf-Hausner, U., Patocchi-Tenzer, I., Angres, B., & Braum, S. (2014). Automation of 3D cell culture using chemically defined hydrogels. SLAS Technology: Translating Life Sciences Innovation, 19(2), 191–197. https://doi.org/10.1177/2211068213508651

Rimann, M. et al. (2014) ‘Automation of 3D cell culture using chemically defined hydrogels’, SLAS Technology: Translating Life Sciences Innovation, 19(2), pp. 191–197. Available at: https://doi.org/10.1177/2211068213508651.

M. Rimann, U. Graf-Hausner, I. Patocchi-Tenzer, B. Angres, and S. Braum, “Automation of 3D cell culture using chemically defined hydrogels,” SLAS Technology: Translating Life Sciences Innovation, vol. 19, no. 2, pp. 191–197, 2014, doi: 10.1177/2211068213508651.

RIMANN, Markus, Ursula GRAF-HAUSNER, Isabel PATOCCHI-TENZER, Brigitte ANGRES und Susanne BRAUM, 2014. Automation of 3D cell culture using chemically defined hydrogels. SLAS Technology: Translating Life Sciences Innovation. 2014. Bd. 19, Nr. 2, S. 191–197. DOI 10.1177/2211068213508651

Rimann, Markus, Ursula Graf-Hausner, Isabel Patocchi-Tenzer, Brigitte Angres, and Susanne Braum. 2014. “Automation of 3D Cell Culture Using Chemically Defined Hydrogels.” SLAS Technology: Translating Life Sciences Innovation 19 (2): 191–97. https://doi.org/10.1177/2211068213508651.

Rimann, Markus, et al. “Automation of 3D Cell Culture Using Chemically Defined Hydrogels.” SLAS Technology: Translating Life Sciences Innovation, vol. 19, no. 2, 2014, pp. 191–97, https://doi.org/10.1177/2211068213508651.