Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3

Ruinelli, Michela; Schneeberger, Pierre H.H; Ferrante, Patrizia; Bühlmann, Andreas; Scortichini, Marco; Vanneste, Joel L.; Duffy, Brion; Pothier, Joël F.

doi:10.1111/ppa.12551

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3
Authors:	Ruinelli, Michela Schneeberger, Pierre H.H Ferrante, Patrizia Bühlmann, Andreas Scortichini, Marco Vanneste, Joel L. Duffy, Brion Pothier, Joël F.
DOI:	10.1111/ppa.12551
Published in:	Plant Pathology
Volume(Issue):	66
Issue:	1
Page(s):	140
Pages to:	149
Issue Date:	6-Jun-2016
Publisher / Ed. Institution:	Wiley
ISSN:	0032-0862 1365-3059
Language:	English
Subjects:	Field based detection; Actinidiae
Subject (DDC):	570: Biology
Abstract:	The aim of this study was to develop a rapid, sensitive and reliable field-based assay for detection of the quarantine pathogen Pseudomonas syringae pv. actinidiae, the causal agent of the most destructive and economically important bacterial disease of kiwifruit. A comparative genomic approach was used on the publicly available P. syringae pv. actinidiae genomic data to select unique target regions for the development of two loop-mediated isothermal amplification (LAMP) assays able to detect P. syringae pv. actinidiae and to discriminate strains belonging to the highly virulent and globally spreading P. syringae pv. actinidiae biovar 3. Both LAMP assays showed specificity in accordance to their target and were able to detect reliably 125 CFU per reaction in less than 30 min. The developed assays were able to detect the presence of P. syringae pv. actinidiae in symptomatic as well as in asymptomatic naturally infected kiwifruit material, thus increasing the potential of the LAMP assays for phytosanitary purposes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1640
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	DROPSA: Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens
Appears in collections:	Publikationen Life Sciences und Facility Management

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Ruinelli, M., Schneeberger, P. H. H., Ferrante, P., Bühlmann, A., Scortichini, M., Vanneste, J. L., Duffy, B., & Pothier, J. F. (2016). Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3. Plant Pathology, 66(1), 140–149. https://doi.org/10.1111/ppa.12551

Ruinelli, M. et al. (2016) ‘Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3’, Plant Pathology, 66(1), pp. 140–149. Available at: https://doi.org/10.1111/ppa.12551.

M. Ruinelli et al., “Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3,” Plant Pathology, vol. 66, no. 1, pp. 140–149, Jun. 2016, doi: 10.1111/ppa.12551.

RUINELLI, Michela, Pierre H.H SCHNEEBERGER, Patrizia FERRANTE, Andreas BÜHLMANN, Marco SCORTICHINI, Joel L. VANNESTE, Brion DUFFY und Joël F. POTHIER, 2016. Comparative genomics-informed design of two LAMP detection assays for detection of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae and discrimination of isolates belonging to the pandemic biovar 3. Plant Pathology. 6 Juni 2016. Bd. 66, Nr. 1, S. 140–149. DOI 10.1111/ppa.12551

Ruinelli, Michela, Pierre H.H Schneeberger, Patrizia Ferrante, Andreas Bühlmann, Marco Scortichini, Joel L. Vanneste, Brion Duffy, and Joël F. Pothier. 2016. “Comparative Genomics-Informed Design of Two LAMP Detection Assays for Detection of the Kiwifruit Pathogen Pseudomonas Syringae Pv. Actinidiae and Discrimination of Isolates Belonging to the Pandemic Biovar 3.” Plant Pathology 66 (1): 140–49. https://doi.org/10.1111/ppa.12551.

Ruinelli, Michela, et al. “Comparative Genomics-Informed Design of Two LAMP Detection Assays for Detection of the Kiwifruit Pathogen Pseudomonas Syringae Pv. Actinidiae and Discrimination of Isolates Belonging to the Pandemic Biovar 3.” Plant Pathology, vol. 66, no. 1, June 2016, pp. 140–49, https://doi.org/10.1111/ppa.12551.