Xanthomonas arboricola pv. fragariae : what's in a name?

Vandroemme, Joachim; Cottyn, Bart; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Maes, Martine

doi:10.1111/ppa.12028

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Xanthomonas arboricola pv. fragariae : what's in a name?
Authors:	Vandroemme, Joachim Cottyn, Bart Pothier, Joël F. Pflüger, Valentin Duffy, Brion Maes, Martine
DOI:	10.1111/ppa.12028
Published in:	Plant Pathology
Volume(Issue):	62
Issue:	5
Page(s):	1123
Pages to:	1131
Issue Date:	Oct-2013
Publisher / Ed. Institution:	Wiley
ISSN:	0032-0862 1365-3059
Language:	English
Subject (DDC):	570: Biology
Abstract:	Polyphasic analysis exposed important heterogeneity between bacterial strains catalogued as Xanthomonas arboricola pv. fragariae (Xaf) from different culture collections. Two draft whole-genome sequences revealed pathogenicity related genes of the type-three secretion system in strain LMG 19146, whereas none were found in the Xaf pathotype strain LMG 19145. Also, considerable sequence divergence was observed in the phylogenetic marker genes gyrB, rpoD, dnaK and fyuA. Further study of 16 Xaf culture-collection strains showed that co-classification is not justified. Partial 16S rRNA gene and gyrB sequencing demonstrated that 12 strains belonged to X. arboricola, but that they did not form one homogeneous group within the species. The four remaining strains were identified as Xanthomonas fragariae and Xanthomonas sp. All sequence-based identifications were confirmed by MALDI-TOF MS fingerprinting. Also, the pathogenicity genes hrcQ and avrBs2 were detected in only three of the 12 analysed X. arboricola strains. The X. arboricola and Xanthomonas sp. strains showed pectolytic activity, and upon inoculation in strawberry none of the strains reproduced the leaf blight symptoms reported for Xaf. This study demonstrates that (i) no clear criteria exist for the identification of strains as Xaf, (ii) the name Xaf is currently used for a genetically diverse assortment of strains, and (iii) the species X. arboricola holds many undetermined plant-associated bacteria besides the described pathovars.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1644
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads
Appears in collections:	Publikationen Life Sciences und Facility Management

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Vandroemme, J., Cottyn, B., Pothier, J. F., Pflüger, V., Duffy, B., & Maes, M. (2013). Xanthomonas arboricola pv. fragariae : what’s in a name? Plant Pathology, 62(5), 1123–1131. https://doi.org/10.1111/ppa.12028

Vandroemme, J. et al. (2013) ‘Xanthomonas arboricola pv. fragariae : what’s in a name?’, Plant Pathology, 62(5), pp. 1123–1131. Available at: https://doi.org/10.1111/ppa.12028.

J. Vandroemme, B. Cottyn, J. F. Pothier, V. Pflüger, B. Duffy, and M. Maes, “Xanthomonas arboricola pv. fragariae : what’s in a name?,” Plant Pathology, vol. 62, no. 5, pp. 1123–1131, Oct. 2013, doi: 10.1111/ppa.12028.

VANDROEMME, Joachim, Bart COTTYN, Joël F. POTHIER, Valentin PFLÜGER, Brion DUFFY und Martine MAES, 2013. Xanthomonas arboricola pv. fragariae : what’s in a name? Plant Pathology. Oktober 2013. Bd. 62, Nr. 5, S. 1123–1131. DOI 10.1111/ppa.12028

Vandroemme, Joachim, Bart Cottyn, Joël F. Pothier, Valentin Pflüger, Brion Duffy, and Martine Maes. 2013. “Xanthomonas Arboricola Pv. Fragariae : What’s in a Name?” Plant Pathology 62 (5): 1123–31. https://doi.org/10.1111/ppa.12028.

Vandroemme, Joachim, et al. “Xanthomonas Arboricola Pv. Fragariae : What’s in a Name?” Plant Pathology, vol. 62, no. 5, Oct. 2013, pp. 1123–31, https://doi.org/10.1111/ppa.12028.