Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry

Kałużna, Monika; Willems, Anne; Pothier, Joël F.; Ruinelli, Michela; Sobiczewski, Piotr; Puławska, Joanna

doi:10.1016/j.syapm.2016.05.005

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry
Authors:	Kałużna, Monika Willems, Anne Pothier, Joël F. Ruinelli, Michela Sobiczewski, Piotr Puławska, Joanna
DOI:	10.1016/j.syapm.2016.05.005
Published in:	Systematic and Applied Microbiology
Volume(Issue):	39
Issue:	6
Page(s):	370
Pages to:	377
Issue Date:	Sep-2016
Publisher / Ed. Institution:	Elsevier
Publisher / Ed. Institution:	Amsterdam
ISSN:	0723-2020 1618-0984
Language:	English
Subjects:	Pseudomonas sp; Cherry bacterial canker; DNA–DNA hybridization; Genome; MLSA; MALDI-TOF MS; FAME
Subject (DDC):	570: Biology
Abstract:	Eight isolates of Gram-negative fluorescent bacteria (58T, 122, 374, 791, 963, 966, 970a and 1021) were obtained from diseased tissue of cherry trees from different regions of Poland. The symptoms resembled those of bacterial canker. Based on an analysis of 16S rDNA sequences the isolates shared the highest over 99.9% similarity with Pseudomonas ficuserectae JCM 2400T and Pseudomonas congelans DSM 14939T. Phylogenetic analysis using housekeeping genes gyrB, rpoD and rpoB revealed that they form a separate cluster and confirmed their closest relation to P. syringae NCPPB 281T and P. congelans LMG 21466T. DNA–DNA hybridization between the cherry isolate 58T and the type strains of these two closely related species revealed relatedness values of 58.2% and 41.9%, respectively. This was further supported by Average Nucleotide Identity (ANIb) and Genome-to-Genome Distance (GGDC) between the whole genome sequences of strain LMG 28609T and closely related Pseudomonas species. The major cellular fatty acids are 16:0 and summed feature 3 (16:1 ω7c/15:0 iso 2OH). Phenotypic characteristics differentiated the novel isolates from other closely related species. The G + C content of the genomic DNA of strain 58T was 59%. The diversity was proved by PCR MP and BOX PCR, eliminating the possibility that they constitute a clonal population. Based on the evidence of this polyphasic taxonomic study the eight strains are considered to represent a novel species of the genus Pseudomonas for which the name Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed. The type strain of this species is 58T (= LMG 28609T = CFBP 8305T).
URI:	https://digitalcollection.zhaw.ch/handle/11475/1663
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	COST Action FA1104 – Sustainable production of high quality cherries for the European market
Appears in collections:	Publikationen Life Sciences und Facility Management

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Kałużna, M., Willems, A., Pothier, J. F., Ruinelli, M., Sobiczewski, P., & Puławska, J. (2016). Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry. Systematic and Applied Microbiology, 39(6), 370–377. https://doi.org/10.1016/j.syapm.2016.05.005

Kałużna, M. et al. (2016) ‘Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry’, Systematic and Applied Microbiology, 39(6), pp. 370–377. Available at: https://doi.org/10.1016/j.syapm.2016.05.005.

M. Kałużna, A. Willems, J. F. Pothier, M. Ruinelli, P. Sobiczewski, and J. Puławska, “Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry,” Systematic and Applied Microbiology, vol. 39, no. 6, pp. 370–377, Sep. 2016, doi: 10.1016/j.syapm.2016.05.005.

KAŁUŻNA, Monika, Anne WILLEMS, Joël F. POTHIER, Michela RUINELLI, Piotr SOBICZEWSKI und Joanna PUŁAWSKA, 2016. Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry. Systematic and Applied Microbiology. September 2016. Bd. 39, Nr. 6, S. 370–377. DOI 10.1016/j.syapm.2016.05.005

Kałużna, Monika, Anne Willems, Joël F. Pothier, Michela Ruinelli, Piotr Sobiczewski, and Joanna Puławska. 2016. “Pseudomonas Cerasi Sp. Nov. (Non Griffin, 1911) Isolated from Diseased Tissue of Cherry.” Systematic and Applied Microbiology 39 (6): 370–77. https://doi.org/10.1016/j.syapm.2016.05.005.

Kałużna, Monika, et al. “Pseudomonas Cerasi Sp. Nov. (Non Griffin, 1911) Isolated from Diseased Tissue of Cherry.” Systematic and Applied Microbiology, vol. 39, no. 6, Sept. 2016, pp. 370–77, https://doi.org/10.1016/j.syapm.2016.05.005.