Screening for active substances with TLC bioautography : challenges with artefacts

Merki, Rebekka; Schönborn, Andreas; Pihan, Lisa-Anna-Maria; Wolfram, Evelyn

Publication type:	Conference poster
Type of review:	Peer review (abstract)
Title:	Screening for active substances with TLC bioautography : challenges with artefacts
Authors:	Merki, Rebekka Schönborn, Andreas Pihan, Lisa-Anna-Maria Wolfram, Evelyn
et. al:	No
Proceedings:	12th BioDetectors Conference 2019 : Abstract book
Conference details:	12th BioDetectors Conference 2019, Vienna, Austria, 26-27 September 2019
Issue Date:	2019
Publisher / Ed. Institution:	BioDetection Systems
Publisher / Ed. Institution:	Vienna
Language:	English
Subject (DDC):	540: Chemistry
Abstract:	TLC, and its modern form HPTLC, is a standard method for identification and quality control of herbals [1]. Its combination with bioassays offers simultaneous separation and screening of plant extracts and other multi-compound mixtures for active substances. The intriguing simplicity and rapidness of the method might lead to underestimation of potential artefacts, first reported by Taibon et al. for HPTLC-Tyrosinase Inhibition [2]. In assays based on cells or enzymes in hydrophilic media, high lipophilic substances prevent contact with the potentially active substance and might lead to false-positive or false-negative results. Additionally, diffusion problems from the layer to the biological assay agents can also lead to wrong interpretation of results, due to difference in the physico-chemical properties of the molecules in the sample mixture. The planar Yeast Estrogen Screen Assay (pYES) is used for detection of human estrogen receptor alpha agonists in mixtures [3]. The hER-alpha is expressing beta-galactosidase in presence of estrogenic substances. Substances with a strong blue autofluorescence at 366 nm detection and low estrogenic activity are difficult to differentiate from real estrogenic activity, when using the blue fluorescent dye 4-methylumbelliferyl-beta-D-galactopyranosid (MUG) as substarte for beta galactosidase. Results of blue fluorescing phenolic acid rich plant extracts with known estrogen activity are demonstrated and a control assay using beta-D-galactopyranoside (RGP) as substrate is proposed [4]. The poster summarizes potential artefacts from pYES and other antimicrobial assays and reviews the theoretical considerations and suggests further investigation to overcome the challenges and improve bioautographic methods.
URI:	https://digitalcollection.zhaw.ch/handle/11475/19502
Fulltext version:	Published version
License (according to publishing contract):	Not specified
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Merki, R., Schönborn, A., Pihan, L.-A.-M., & Wolfram, E. (2019). Screening for active substances with TLC bioautography : challenges with artefacts. 12th BioDetectors Conference 2019 : Abstract Book.

Merki, R. et al. (2019) ‘Screening for active substances with TLC bioautography : challenges with artefacts’, in 12th BioDetectors Conference 2019 : Abstract book. Vienna: BioDetection Systems.

R. Merki, A. Schönborn, L.-A.-M. Pihan, and E. Wolfram, “Screening for active substances with TLC bioautography : challenges with artefacts,” in 12th BioDetectors Conference 2019 : Abstract book, 2019.

MERKI, Rebekka, Andreas SCHÖNBORN, Lisa-Anna-Maria PIHAN und Evelyn WOLFRAM, 2019. Screening for active substances with TLC bioautography : challenges with artefacts. In: 12th BioDetectors Conference 2019 : Abstract book. Conference poster. Vienna: BioDetection Systems. 2019

Merki, Rebekka, Andreas Schönborn, Lisa-Anna-Maria Pihan, and Evelyn Wolfram. 2019. “Screening for Active Substances with TLC Bioautography : Challenges with Artefacts.” Conference poster. In 12th BioDetectors Conference 2019 : Abstract Book. Vienna: BioDetection Systems.

Merki, Rebekka, et al. “Screening for Active Substances with TLC Bioautography : Challenges with Artefacts.” 12th BioDetectors Conference 2019 : Abstract Book, BioDetection Systems, 2019.