Recent advances in effect-directed enzyme assays based on thin-layer chromatography

Wolfram, Evelyn; Bräm, Sarah

doi:10.1002/pca.2669

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Recent advances in effect-directed enzyme assays based on thin-layer chromatography
Authors:	Wolfram, Evelyn Bräm, Sarah
DOI:	10.1002/pca.2669
Published in:	Phytochemical Analysis
Volume(Issue):	28
Issue:	2
Page(s):	74
Pages to:	86
Issue Date:	1-Feb-2017
Publisher / Ed. Institution:	Wiley
ISSN:	1099-1565 0958-0344
Language:	English
Subjects:	Drug Discovery; Bioautography; Enzyme assay; HPTLC
Subject (DDC):	572: Biochemistry 580: Plants (Botany) 660.6: Biotechnology
Abstract:	Thin-layer chromatography (TLC) together with its more modern form HPTLC is a rapid and cost effective analytical tool with a long tradition in quality control of medicinal plants, extracts and natural products. Separated compounds are fixed on the solid silica phase to form a compound library. Through direct coupling of visualizable enzyme reactions on the TLC plate, this compound library can also be used for activity screening. Such TLC-based bioautographic enzyme and enzyme inhibition assays complement first stage development activity screening assays. They provide not only phytochemical results by chromatographic separation, but also additional information about the activity of constituents or fractions in multi-compound mixtures, and thus can reveal and distinguish artefacts generated by certain compound classes. This review summarizes recently introduced TLC bioautographic enzyme assays as well as advances in already existing procedures. Bioautographic enzyme and enzyme inhibitory assays offer a rapid, high-throughput method for screening of secondary metabolite profiles for potential enzyme and enzyme inhibitory activities.
URI:	https://digitalcollection.zhaw.ch/handle/11475/1959
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Wolfram, E., & Bräm, S. (2017). Recent advances in effect-directed enzyme assays based on thin-layer chromatography. Phytochemical Analysis, 28(2), 74–86. https://doi.org/10.1002/pca.2669

Wolfram, E. and Bräm, S. (2017) ‘Recent advances in effect-directed enzyme assays based on thin-layer chromatography’, Phytochemical Analysis, 28(2), pp. 74–86. Available at: https://doi.org/10.1002/pca.2669.

E. Wolfram and S. Bräm, “Recent advances in effect-directed enzyme assays based on thin-layer chromatography,” Phytochemical Analysis, vol. 28, no. 2, pp. 74–86, Feb. 2017, doi: 10.1002/pca.2669.

WOLFRAM, Evelyn und Sarah BRÄM, 2017. Recent advances in effect-directed enzyme assays based on thin-layer chromatography. Phytochemical Analysis. 1 Februar 2017. Bd. 28, Nr. 2, S. 74–86. DOI 10.1002/pca.2669

Wolfram, Evelyn, and Sarah Bräm. 2017. “Recent Advances in Effect-Directed Enzyme Assays Based on Thin-Layer Chromatography.” Phytochemical Analysis 28 (2): 74–86. https://doi.org/10.1002/pca.2669.

Wolfram, Evelyn, and Sarah Bräm. “Recent Advances in Effect-Directed Enzyme Assays Based on Thin-Layer Chromatography.” Phytochemical Analysis, vol. 28, no. 2, Feb. 2017, pp. 74–86, https://doi.org/10.1002/pca.2669.