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dc.contributor.authorWolfram, Evelyn-
dc.contributor.authorPeter, Samuel-
dc.contributor.authorTodorova, M-
dc.contributor.authorTrendafilova, A-
dc.contributor.authorMotyka, V-
dc.contributor.authorDobrev, P-
dc.contributor.authorDanova, Kalina-
dc.date.accessioned2018-01-10T13:02:10Z-
dc.date.available2018-01-10T13:02:10Z-
dc.date.issued2017-10-24-
dc.identifier.issn2509-6656de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1967-
dc.description.abstractCell aggregate lines of Artemisia alba were selected and developed in liquid cultures: ER_3 [0.1 mg/l N 6-benzyladenine (BA) + 1.5 mg/l indole-3-butyric acid (IBA)] and ER_3_NAA [0.1 mg/l BA + 1.5 mg/l naphtylacetic acid (NAA)], grown in the dark, gyratory shaker, 100 rpm; ER_3_hν and ER_3_NAA_hν were grown in the same conditions, but at 16/8h photoperiod. Spectrophotometric assay of the total phenolic and flavonoid compounds was performed and the samples were also tested by HPTLC analysis with chlorogenic acid, as well as 1,3-, 1,5-, 3,5-, 3,4- and 4,5-dicaffeoylquinic acids, scopoletin and fraxidin-8-glucoside as reference compounds. The analysis showed general similarity in the tested samples. Photoperiod, however seemed to have an influence on glucosylation of the samples. While dark grown suspensions exhibited biosynthesis of both the aglycons (scopoletin and fraxidin) as well as their respective glucosides (scopolin and fraxidin glucoside), the 16/8 photoperiod suspensions produced predominantly the glucosylated derivatives. These data were confirmed also by the UHPLC analysis. 3,5-dicaffeoylquinic acid was the predominant component in all samples. In terms of endogenous cytokinin production, a significant drop of their levels was observed in suspensions, where NAA represented the exogenously added auxin. The latter samples were also characterized by a significant stimulation of polyphenolics, comparable to those produced by the differentiated shoot cultures of the plant. The obtained results are of practical interest for the targeted biotechnological delivery of scopoletin and fraxidin, which represent a higher phytopharmacological potential as compared with their glucosylated derivatives.de_CH
dc.language.isoende_CH
dc.publisherThiemede_CH
dc.relation.ispartofPlanta Medica International Opende_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc572: Biochemiede_CH
dc.subject.ddc580: Pflanzen (Botanik)de_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleEffect of growth regulators and photoperiod on endogenous phytohormonal levels and polyphenolic production in Artemisa alba cell aggregate culturesde_CH
dc.typeKonferenz: Posterde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1055/s-0037-1608367de_CH
zhaw.conference.details65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017), Basel, 3-6 September 2017de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume4de_CH
zhaw.publication.reviewPeer review (Abstract)de_CH
zhaw.funding.snfIZEBZO_142989de_CH
zhaw.webfeedPhytopharmaziede_CH
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Wolfram, E., Peter, S., Todorova, M., Trendafilova, A., Motyka, V., Dobrev, P., & Danova, K. (2017). Effect of growth regulators and photoperiod on endogenous phytohormonal levels and polyphenolic production in Artemisa alba cell aggregate cultures [Conference poster]. Planta Medica International Open, 4. https://doi.org/10.1055/s-0037-1608367
Wolfram, E. et al. (2017) ‘Effect of growth regulators and photoperiod on endogenous phytohormonal levels and polyphenolic production in Artemisa alba cell aggregate cultures’, in Planta Medica International Open. Thieme. Available at: https://doi.org/10.1055/s-0037-1608367.
E. Wolfram et al., “Effect of growth regulators and photoperiod on endogenous phytohormonal levels and polyphenolic production in Artemisa alba cell aggregate cultures,” in Planta Medica International Open, Oct. 2017, vol. 4. doi: 10.1055/s-0037-1608367.
WOLFRAM, Evelyn, Samuel PETER, M TODOROVA, A TRENDAFILOVA, V MOTYKA, P DOBREV und Kalina DANOVA, 2017. Effect of growth regulators and photoperiod on endogenous phytohormonal levels and polyphenolic production in Artemisa alba cell aggregate cultures. In: Planta Medica International Open. Conference poster. Thieme. 24 Oktober 2017
Wolfram, Evelyn, Samuel Peter, M Todorova, A Trendafilova, V Motyka, P Dobrev, and Kalina Danova. 2017. “Effect of Growth Regulators and Photoperiod on Endogenous Phytohormonal Levels and Polyphenolic Production in Artemisa Alba Cell Aggregate Cultures.” Conference poster. In Planta Medica International Open. Vol. 4. Thieme. https://doi.org/10.1055/s-0037-1608367.
Wolfram, Evelyn, et al. “Effect of Growth Regulators and Photoperiod on Endogenous Phytohormonal Levels and Polyphenolic Production in Artemisa Alba Cell Aggregate Cultures.” Planta Medica International Open, vol. 4, Thieme, 2017, https://doi.org/10.1055/s-0037-1608367.


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