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https://doi.org/10.21256/zhaw-21024| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Transcriptome analysis of Xanthomonas fragariae in strawberry leaves |
| Authors: | Puławska, Joanna Kałużna, Monika Warabieda, Wojciech Pothier, Joël F. Gétaz, Michael van der Wolf, Jan M. |
| et. al: | No |
| DOI: | 10.1038/s41598-020-77612-y 10.21256/zhaw-21024 |
| Published in: | Scientific Reports |
| Volume(Issue): | 10 |
| Issue: | 20582 |
| Issue Date: | 25-Nov-2020 |
| Publisher / Ed. Institution: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| Language: | English |
| Subjects: | Xanthomonas; Transcriptome; RNA-seq; Strawberry |
| Subject (DDC): | 570: Biology |
| Abstract: | Xanthomonas fragariae is a quarantine bacterial pathogen that causes angular leaf spot on strawberry. The aim of our study was to analyse the mechanism of interaction of this bacterium with its host plant at the transcriptome level. For this purpose, mRNAs of X. fragariae growing in Wilbrink's medium and from infected strawberry cv. Elsanta plants were isolated and sequenced using the Illumina MiSeq platform. The expression profiles of the bacteria in Wilbrink's medium and in planta were very diverse. Of the 3939 CDSs recorded, 1995 had significantly different expression in planta (966 and 1029 genes were down- and upregulated, respectively). Among the genes showing increased expression in planta, those with eggNOG/COG (evolutionary genealogy of genes: Non-supervised Orthologous Groups/Cluster of Orthologous Groups) categories associated with bacterial cell motility, signal transduction, transport and metabolism of inorganic ions and carbohydrates and transcription were overrepresented. Among the genes with the most increased expression in planta, genes primarily associated with flagella synthesis and chemotaxis were found. It is also interesting to note that out of the 31 genes localized on a plasmid, 16 were expressed differently in planta, which may indicate their potential role in plant-pathogen interactions. Many genes with differentiated expression that were localized on chromosome and plasmid encode proteins of unknown function. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/21024 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | DROPSA – Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2020_Pulawska-etal_Transcriptome-analysis-Xanthomonas-fragariae_Scientific-Reports.pdf | Article | 1.26 MB | Adobe PDF |  View/Open |
| 2020_Pulawska-etal_Transcriptome-analysis_Supplement.pdf | Supplementary Materials | 1.09 MB | Adobe PDF |  View/Open |
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Puławska, J., Kałużna, M., Warabieda, W., Pothier, J. F., Gétaz, M., & van der Wolf, J. M. (2020). Transcriptome analysis of Xanthomonas fragariae in strawberry leaves. Scientific Reports, 10(20582). https://doi.org/10.1038/s41598-020-77612-y
Puławska, J. et al. (2020) ‘Transcriptome analysis of Xanthomonas fragariae in strawberry leaves’, Scientific Reports, 10(20582). Available at: https://doi.org/10.1038/s41598-020-77612-y.
J. Puławska, M. Kałużna, W. Warabieda, J. F. Pothier, M. Gétaz, and J. M. van der Wolf, “Transcriptome analysis of Xanthomonas fragariae in strawberry leaves,” Scientific Reports, vol. 10, no. 20582, Nov. 2020, doi: 10.1038/s41598-020-77612-y.
PUŁAWSKA, Joanna, Monika KAŁUŻNA, Wojciech WARABIEDA, Joël F. POTHIER, Michael GÉTAZ und Jan M. VAN DER WOLF, 2020. Transcriptome analysis of Xanthomonas fragariae in strawberry leaves. Scientific Reports. 25 November 2020. Bd. 10, Nr. 20582. DOI 10.1038/s41598-020-77612-y
Puławska, Joanna, Monika Kałużna, Wojciech Warabieda, Joël F. Pothier, Michael Gétaz, and Jan M. van der Wolf. 2020. “Transcriptome Analysis of Xanthomonas Fragariae in Strawberry Leaves.” Scientific Reports 10 (20582). https://doi.org/10.1038/s41598-020-77612-y.
Puławska, Joanna, et al. “Transcriptome Analysis of Xanthomonas Fragariae in Strawberry Leaves.” Scientific Reports, vol. 10, no. 20582, Nov. 2020, https://doi.org/10.1038/s41598-020-77612-y.
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