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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Development of a biodegradable microcarrier for the cultivation of human adipose stem cells (hASCs) with a defined xeno- and serum-free medium
Authors: Muoio, Francesco
Panella, Stefano
Lindner, Matias
Jossen, Valentin
Harder, Yves
Moccetti, Tiziano
Eibl-Schindler, Regine
Müller, Michele
Tallone, Tiziano
et. al: No
DOI: 10.3390/app11030925
Published in: Applied Sciences
Volume(Issue): 11
Issue: 3
Page(s): 925
Issue Date: 20-Jan-2021
Publisher / Ed. Institution: MDPI
ISSN: 2076-3417
Language: English
Subjects: Xeno- and serum-free cell culture medium; UrSuppe; Bioresorbable/biodegradable; Microcarrier (MC); Human adipose stem cells (hASCs)
Subject (DDC): 660.6: Biotechnology
Abstract: Stirred single-use bioreactors in combination with microcarriers (MCs) have established themselves as a technology that has the potential to meet the demands of current and future cell therapeutic markets. However, most of the published processes have been performed using fetal bovine serum (FBS) containing cell culture medium and non-biocompatible MCs. This approach has two significant drawbacks: firstly, the inevitable potential risks associated with the use of FBS for clinical applications; secondly, non-biocompatible MCs have to be removed from the cell suspension before implantation, requiring a step that causes loss of viable cells and adds further costs and complications. This study aimed to develop a new platform based on a chemically defined xeno- and serum-free cell culture medium and biodegradable MC that can support the growth of human adipose stem cells (hASCs) while still preserving their undifferentiated status. A specific combination of components and manufacturing parameters resulted in a MC prototype, called “BR44”, which delivered the desired functionality. MC BR44 allows the hASCs to stick to its surface and grow in a chemically defined xeno- and serum-free medium (UrSuppe). Although the cells’ expansion rate was not as high as with a commercial non-biodegradable standard MC, those cultured on BR44 maintained a better undifferentiated status in both static and dynamic conditions than those cultured on traditional 2D surfaces.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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