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dc.contributor.authorJossen, Valentin-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorKraume, Matthias-
dc.contributor.authorEibl, Dieter-
dc.date.accessioned2021-03-15T12:49:14Z-
dc.date.available2021-03-15T12:49:14Z-
dc.date.issued2018-09-
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/22070-
dc.description.abstractHuman adipose tissue-derived stromal/stem cells (hASC) represent a valuable source of cells for clinical applications, especially in the field of regenerative medicine. Therefore, it comes as no surprise that interest in hASCs has increased greatly over the last decade. However, in order to use hASCs successfully in clinical applications, in vitro expansion is required. Single-use bioreactors in combination with microcarriers (MC) have been shown to be suitable systems for this task (1-3). However, hASCs are prone to higher shear sensitivity than conventional cell lines (e.g. CHO, BHK) that are normally expanded in these systems. Hence, the goal of this study was to investigate the influence of different shear stress levels on the growth of hASCs in small scale single-use spinner flasks. For this purpose, Computational Fluid Dynamics simulations based on a Euler-Euler and Euler-Lagrange approach were performed to predict the hydrodynamic stresses (0.06 – 0.87 Pa), the residence times (0.4 – 7.3 s) and the circulation times (1.6 - 16.6 s) of the MCs in various high shear zones. The numerical findings were combined with experimental data from cultivation studies (0.29 – 1.1∙106 hASC/mL) in order to develop a segregated mathematical growth model for the prediction of MC-associated hASC growth in small scale single-use spinner flasks.de_CH
dc.language.isoende_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleGrowth behavior of human adipose tissue-derived stromal/stem cells in single-use spinner flasks : numerical and experimental investigationsde_CH
dc.typeKonferenz: Posterde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.conference.detailsECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.publication.reviewNot specifiedde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Jossen, V., Eibl-Schindler, R., Kraume, M., & Eibl, D. (2018, September). Growth behavior of human adipose tissue-derived stromal/stem cells in single-use spinner flasks : numerical and experimental investigations. ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018.
Jossen, V. et al. (2018) ‘Growth behavior of human adipose tissue-derived stromal/stem cells in single-use spinner flasks : numerical and experimental investigations’, in ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018.
V. Jossen, R. Eibl-Schindler, M. Kraume, and D. Eibl, “Growth behavior of human adipose tissue-derived stromal/stem cells in single-use spinner flasks : numerical and experimental investigations,” in ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018, Sep. 2018.
JOSSEN, Valentin, Regine EIBL-SCHINDLER, Matthias KRAUME und Dieter EIBL, 2018. Growth behavior of human adipose tissue-derived stromal/stem cells in single-use spinner flasks : numerical and experimental investigations. In: ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018. Conference poster. September 2018
Jossen, Valentin, Regine Eibl-Schindler, Matthias Kraume, and Dieter Eibl. 2018. “Growth Behavior of Human Adipose Tissue-Derived Stromal/Stem Cells in Single-Use Spinner Flasks : Numerical and Experimental Investigations.” Conference poster. In ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018.
Jossen, Valentin, et al. “Growth Behavior of Human Adipose Tissue-Derived Stromal/Stem Cells in Single-Use Spinner Flasks : Numerical and Experimental Investigations.” ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018, 2018.


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