Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kremer, Antje | - |
dc.contributor.author | Wussmann, Maximiliane | - |
dc.contributor.author | Herrmann, Marietta | - |
dc.contributor.author | Raghunath, Michael | - |
dc.contributor.author | Walles, Heike | - |
dc.date.accessioned | 2021-03-26T15:17:24Z | - |
dc.date.available | 2021-03-26T15:17:24Z | - |
dc.date.issued | 2019-12-17 | - |
dc.identifier.issn | 1386-0291 | de_CH |
dc.identifier.issn | 1875-8622 | de_CH |
dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/22186 | - |
dc.description.abstract | Background: Prolyl hydroxylase inhibitors (PHIs) are promising compounds to promote angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α), a master regulator of angiogenesis. Increased HIF-1α presence induces expression of proangiogenic genes such as vascular endothelial growth factor (VEGF). Objective:We investigated the pharmacological induction of hypoxia via the PHI ciclopirox olamine (CPX) as angiogenesis strategy on human dermal microvascular endothelial cell (hd-mvEC) spheroids directly and indirectly via activating human mesenchymal stem cells (hMSCs). Methods: HMSCs were isolated from bone marrow and hd-mvECs from foreskin biopsies. MSC-conditioned medium after CPX stimulation (MSC-CM CPX) was analyzed by VEGF ELISA and Proteome Profiler™ Human Angiogenesis Array. Direct stimulation with CPX and indirect stimulation via MSC-CM CPX were compared in sprouting assays of hd-mvEC spheroids. Results: Direct stimulation with CPX significantly increased sprouting of hd-mvEC spheroids. MSC-CM CPX also induced sprouting from hd-mvEC spheroids, which was mediated by angiogenic VEGF and other proangiogenic factors that had been produced by stimulated hMSCs. Conclusions: The stimulation with CPX increased the proangiogenic response of hd-mvECs and hMSCs. The direct stimulation of hd-mvECs with CPX has the potential to replace external VEGF supplementation. Thus, CPX can induce angiogenesis in ECs even in the absence of auxiliary cells demonstrating a promising proangiogenic approach. | de_CH |
dc.language.iso | en | de_CH |
dc.publisher | IOS Press | de_CH |
dc.relation.ispartof | Clinical Hemorheology and Microcirculation | de_CH |
dc.rights | Licence according to publishing contract | de_CH |
dc.subject | HIF-1α | de_CH |
dc.subject | Angiogenesis | de_CH |
dc.subject | Ciclopirox olamine | de_CH |
dc.subject | Endothelial cells | de_CH |
dc.subject | Mesenchymal stem cells | de_CH |
dc.subject | Prolyl hydroxylase inhibitor | de_CH |
dc.subject | Sprouting | de_CH |
dc.subject | Vascularization | de_CH |
dc.subject | Ciclopirox | de_CH |
dc.subject | Gene expression | de_CH |
dc.subject | Human | de_CH |
dc.subject | Pathologic neovascularization | de_CH |
dc.subject.ddc | 610: Medizin und Gesundheit | de_CH |
dc.title | Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells | de_CH |
dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
dcterms.type | Text | de_CH |
zhaw.departement | Life Sciences und Facility Management | de_CH |
zhaw.organisationalunit | Institut für Chemie und Biotechnologie (ICBT) | de_CH |
dc.identifier.doi | 10.3233/CH-190559 | de_CH |
dc.identifier.pmid | 31006674 | de_CH |
zhaw.funding.eu | No | de_CH |
zhaw.issue | 2 | de_CH |
zhaw.originated.zhaw | Yes | de_CH |
zhaw.pages.end | 328 | de_CH |
zhaw.pages.start | 317 | de_CH |
zhaw.publication.status | publishedVersion | de_CH |
zhaw.volume | 73 | de_CH |
zhaw.publication.review | Peer review (Publikation) | de_CH |
zhaw.author.additional | No | de_CH |
zhaw.display.portrait | Yes | de_CH |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Kremer, A., Wussmann, M., Herrmann, M., Raghunath, M., & Walles, H. (2019). Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation, 73(2), 317–328. https://doi.org/10.3233/CH-190559
Kremer, A. et al. (2019) ‘Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells’, Clinical Hemorheology and Microcirculation, 73(2), pp. 317–328. Available at: https://doi.org/10.3233/CH-190559.
A. Kremer, M. Wussmann, M. Herrmann, M. Raghunath, and H. Walles, “Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells,” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, pp. 317–328, Dec. 2019, doi: 10.3233/CH-190559.
KREMER, Antje, Maximiliane WUSSMANN, Marietta HERRMANN, Michael RAGHUNATH und Heike WALLES, 2019. Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells. Clinical Hemorheology and Microcirculation. 17 Dezember 2019. Bd. 73, Nr. 2, S. 317–328. DOI 10.3233/CH-190559
Kremer, Antje, Maximiliane Wussmann, Marietta Herrmann, Michael Raghunath, and Heike Walles. 2019. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation 73 (2): 317–28. https://doi.org/10.3233/CH-190559.
Kremer, Antje, et al. “Ciclopirox Olamine Promotes the Angiogenic Response of Endothelial Cells and Mesenchymal Stem Cells.” Clinical Hemorheology and Microcirculation, vol. 73, no. 2, Dec. 2019, pp. 317–28, https://doi.org/10.3233/CH-190559.
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