| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor |
| Authors: | Kaiser, Stephan C. Blaschczok, Katharina Eibl, Dieter |
| Published in: | BioProcess International |
| Issue Date: | 18-Aug-2015 |
| Publisher / Ed. Institution: | Informa Life Sciences |
| ISSN: | 1542-6319 |
| Language: | English |
| Subject (DDC): | 660: Chemical engineering |
| Abstract: | This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 × 106 cells/mL were achieved. Recombinant secreted embryonic alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. Maximum SEAP activities of up to 63 U/mL were reached. A novel stirred glass bioreactor suitable for cell culture applications at benchtop scale was designed by Finesse Solutions, Inc. With a maximum working volume of 2.0 L, this bioreactor is controlled by the Finesse G3Lab controller. |
| URI: | http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor https://digitalcollection.zhaw.ch/handle/11475/2281 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Kaiser, S. C., Blaschczok, K., & Eibl, D. (2015). Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor. BioProcess International. http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor
Kaiser, S.C., Blaschczok, K. and Eibl, D. (2015) ‘Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor’, BioProcess International [Preprint]. Available at: http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor.
S. C. Kaiser, K. Blaschczok, and D. Eibl, “Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor,” BioProcess International, Aug. 2015, [Online]. Available: http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor
KAISER, Stephan C., Katharina BLASCHCZOK und Dieter EIBL, 2015. Cultivation of CHO suspension cells and SEAP expression in the finesse glass bioreactor. BioProcess International [online]. 18 August 2015. Verfügbar unter: http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor
Kaiser, Stephan C., Katharina Blaschczok, and Dieter Eibl. 2015. “Cultivation of CHO Suspension Cells and SEAP Expression in the Finesse Glass Bioreactor.” BioProcess International, August. http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor.
Kaiser, Stephan C., et al. “Cultivation of CHO Suspension Cells and SEAP Expression in the Finesse Glass Bioreactor.” BioProcess International, Aug. 2015, http://www.bioprocessintl.com/sponsored-content/cultivation-of-cho-suspension-cells-and-seap-expression-in-the-finesse-glass-bioreactor.
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