Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722

Moretti, Chiaraluce; Rezzonico, Fabio; Orfei, Benedetta; Cortese, Chiara; Moreno‐Pérez, Alba; van den Burg, Harrold A.; Onofri, Andrea; Firrao, Giuseppe; Ramos, Cayo; Smits, Theo H.M.; Buonaurio, Roberto

doi:10.1111/mpp.13105

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-22926

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722
Authors:	Moretti, Chiaraluce Rezzonico, Fabio Orfei, Benedetta Cortese, Chiara Moreno‐Pérez, Alba van den Burg, Harrold A. Onofri, Andrea Firrao, Giuseppe Ramos, Cayo Smits, Theo H.M. Buonaurio, Roberto
et. al:	No
DOI:	10.1111/mpp.13105 10.21256/zhaw-22926
Published in:	Molecular Plant Pathology
Volume(Issue):	22
Issue:	10
Page(s):	1209
Pages to:	1225
Issue Date:	16-Jul-2021
Publisher / Ed. Institution:	Wiley
ISSN:	1364-3703 1464-6722
Language:	English
Subjects:	Pantoea agglomerans; Pseudomonas savastanoi pv. savastanoi; Pseudomonas syringae complex; T3SS; Endophyte; Hypersensitive reaction; Mutant; Olive knot community
Subject (DDC):	579: Microbiology
Abstract:	The endophytic bacterium Pantoea agglomerans DAPP-PG 734 was previously isolated from olive knots caused by infection with Pseudomonas savastanoi pv. savastanoi DAPP-PG 722. Whole-genome analysis of this P. agglomerans strain revealed the presence of a Hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To assess the role of the P. agglomerans T3SS in the interaction with P. savastanoi pv. savastanoi, we generated independent knockout mutants in three Hrp genes of the P. agglomerans DAPP-PG 734 T3SS (hrpJ, hrpN, and hrpY). In contrast to the wildtype control, all three mutants failed to cause a hypersensitive response when infiltrated in tobacco leaves, suggesting that P. agglomerans T3SS is functional and injects effector proteins in plant cells. In contrast to P. savastanoi pv. savastanoi DAPP-PG 722, the wildtype strain P. agglomerans DAPP-PG 734 and its Hrp T3SS mutants did not cause olive knot disease in 1-year-old olive plants. Coinoculation of P. savastanoi pv. savastanoi with P. agglomerans wildtype strains did not significantly change the knot size, while the DAPP-PG 734 hrpY mutant induced a significant decrease in knot size, which could be complemented by providing hrpY on a plasmid. By epifluorescence microscopy and confocal laser scanning microscopy, we found that the localization patterns in knots were nonoverlapping for P. savastanoi pv. savastanoi and P. agglomerans when coinoculated. Our results suggest that suppression of olive plant defences mediated by the Hrp T3SS of P. agglomerans DAPP-PG 734 positively impacts the virulence of P. savastanoi pv. savastanoi DAPP-PG 722.
URI:	https://digitalcollection.zhaw.ch/handle/11475/22926
Fulltext version:	Published version
License (according to publishing contract):	CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Genomik und Transkriptomik von Erwinia und Pantoea
Appears in collections:	Publikationen Life Sciences und Facility Management

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Moretti, C., Rezzonico, F., Orfei, B., Cortese, C., Moreno‐Pérez, A., van den Burg, H. A., Onofri, A., Firrao, G., Ramos, C., Smits, T. H. M., & Buonaurio, R. (2021). Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Molecular Plant Pathology, 22(10), 1209–1225. https://doi.org/10.1111/mpp.13105

Moretti, C. et al. (2021) ‘Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722’, Molecular Plant Pathology, 22(10), pp. 1209–1225. Available at: https://doi.org/10.1111/mpp.13105.

C. Moretti et al., “Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722,” Molecular Plant Pathology, vol. 22, no. 10, pp. 1209–1225, Jul. 2021, doi: 10.1111/mpp.13105.

MORETTI, Chiaraluce, Fabio REZZONICO, Benedetta ORFEI, Chiara CORTESE, Alba MORENO‐PÉREZ, Harrold A. VAN DEN BURG, Andrea ONOFRI, Giuseppe FIRRAO, Cayo RAMOS, Theo H.M. SMITS und Roberto BUONAURIO, 2021. Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Molecular Plant Pathology. 16 Juli 2021. Bd. 22, Nr. 10, S. 1209–1225. DOI 10.1111/mpp.13105

Moretti, Chiaraluce, Fabio Rezzonico, Benedetta Orfei, Chiara Cortese, Alba Moreno‐Pérez, Harrold A. van den Burg, Andrea Onofri, et al. 2021. “Synergistic Interaction between the Type III Secretion System of the Endophytic Bacterium Pantoea Agglomerans DAPP‐PG 734 and the Virulence of the Causal Agent of Olive Knot Pseudomonas Savastanoi Pv. Savastanoi DAPP‐PG 722.” Molecular Plant Pathology 22 (10): 1209–25. https://doi.org/10.1111/mpp.13105.

Moretti, Chiaraluce, et al. “Synergistic Interaction between the Type III Secretion System of the Endophytic Bacterium Pantoea Agglomerans DAPP‐PG 734 and the Virulence of the Causal Agent of Olive Knot Pseudomonas Savastanoi Pv. Savastanoi DAPP‐PG 722.” Molecular Plant Pathology, vol. 22, no. 10, July 2021, pp. 1209–25, https://doi.org/10.1111/mpp.13105.